Bicyclic peptide ligands specific for EphA2

ABSTRACT

The present invention relates to polypeptides which are covalently bound to aromatic molecular scaffolds such that two or more peptide loops are subtended between attachment points to the scaffold. In particular, the invention describes peptides which are high affinity binders of the Eph receptor tyrosine kinase A2 (EphA2). The invention also includes drug conjugates comprising said peptides, conjugated to one or more effector and/or functional groups, to pharmaceutical compositions comprising said peptide ligands and drug conjugates and to the use of said peptide ligands and dmg conjugates in preventing, suppressing or treating a disease or disorder characterised by overexpression of EphA2 in diseased tissue (such as a tumour).

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a U.S. national stage application under 35 U.S.C. § 371 of International Application No. PCT/GB2018/053676, filed Dec. 19, 2018, which claims priority under 35 U.S.C. § 119 to United Kingdom Application No. GB1721265.5, filed Dec. 19, 2017, each of which is incorporated herein by reference in its entirety.

SEQUENCE LISTING

The instant application contains a Sequence Listing which has been submitted electronically in ASCII format and is hereby incorporated by reference in its entirety. Said ASCII copy, created on Jun. 9, 2020, is named 174712.txt and is 98.6 kilobytes in size.

FIELD OF THE INVENTION

The present invention relates to polypeptides which are covalently bound to aromatic molecular scaffolds such that two or more peptide loops are subtended between attachment points to the scaffold. In particular, the invention describes peptides which are high affinity binders of the Eph receptor tyrosine kinase A2 (EphA2). The invention also includes drug conjugates comprising said peptides, conjugated to one or more effector and/or functional groups, to pharmaceutical compositions comprising said peptide ligands and drug conjugates and to the use of said peptide ligands and drug conjugates in preventing, suppressing or treating a disease or disorder characterised by overexpression of EphA2 in diseased tissue (such as a tumour).

BACKGROUND OF THE INVENTION

Cyclic peptides are able to bind with high affinity and target specificity to protein targets and hence are an attractive molecule class for the development of therapeutics. In fact, several cyclic peptides are already successfully used in the clinic, as for example the antibacterial peptide vancomycin, the immunosuppressant drug cyclosporine or the anti-cancer drug octreotide (Driggers et al. (2008), Nat Rev Drug Discov 7 (7), 608-24). Good binding properties result from a relatively large interaction surface formed between the peptide and the target as well as the reduced conformational flexibility of the cyclic structures. Typically, macrocycles bind to surfaces of several hundred square angstrom, as for example the cyclic peptide CXCR4 antagonist CVX15 (400 Å²; Wu et al. (2007), Science 330, 1066-71), a cyclic peptide with the Arg-Gly-Asp motif binding to integrin αVb3 (355 Å²) (Xiong et al. (2002), Science 296 (5565), 151-5) or the cyclic peptide inhibitor upain-1 binding to urokinase-type plasminogen activator (603 Å²; Zhao et al. (2007), J Struct Biol 160 (1), 1-10).

Due to their cyclic configuration, peptide macrocycles are less flexible than linear peptides, leading to a smaller loss of entropy upon binding to targets and resulting in a higher binding affinity. The reduced flexibility also leads to locking target-specific conformations, increasing binding specificity compared to linear peptides. This effect has been exemplified by a potent and selective inhibitor of matrix metalloproteinase 8, (MMP-8) which lost its selectivity over other MMPs when its ring was opened (Cherney et al. (1998), J Med Chem 41 (11), 1749-51). The favorable binding properties achieved through macrocyclization are even more pronounced in multicyclic peptides having more than one peptide ring as for example in vancomycin, nisin and actinomycin.

Different research teams have previously tethered polypeptides with cysteine residues to a synthetic molecular structure (Kemp and McNamara (1985), J. Org. Chem; Timmerman et al. (2005), ChemBioChem). Meloen and co-workers had used tris(bromomethyl)benzene and related molecules for rapid and quantitative cyclisation of multiple peptide loops onto synthetic scaffolds for structural mimicry of protein surfaces (Timmerman et al. (2005), ChemBioChem). Methods for the generation of candidate drug compounds wherein said compounds are generated by linking cysteine containing polypeptides to a molecular scaffold as for example tris(bromomethyl)benzene are disclosed in WO 2004/077062 and WO 2006/078161.

Phage display-based combinatorial approaches have been developed to generate and screen large libraries of bicyclic peptides to targets of interest (Heinis et al. (2009), Nat Chem Biol 5 (7), 502-7 and WO 2009/098450). Briefly, combinatorial libraries of linear peptides containing three cysteine residues and two regions of six random amino acids (Cys-(Xaa)₆-Cys-(Xaa)₆-Cys) were displayed on phage and cyclised by covalently linking the cysteine side chains to a small molecule (tris-(bromomethyl)benzene).

SUMMARY OF THE INVENTION

According to a first aspect of the invention, there is provided a peptide ligand specific for EphA2 comprising a polypeptide comprising at least three cysteine residues, separated by at least two loop sequences, and an aromatic molecular scaffold which forms covalent bonds with the cysteine residues of the polypeptide such that at least two polypeptide loops are formed on the molecular scaffold.

According to a further aspect of the invention, there is provided a drug conjugate comprising a peptide ligand as defined herein conjugated to one or more effector and/or functional groups.

According to a further aspect of the invention, there is provided a pharmaceutical composition comprising a peptide ligand or a drug conjugate as defined herein in combination with one or more pharmaceutically acceptable excipients.

According to a further aspect of the invention, there is provided a peptide ligand or drug conjugate as defined herein for use in preventing, suppressing or treating a disease or disorder characterised by overexpression of EphA2 in diseased tissue (such as a tumour).

DETAILED DESCRIPTION OF THE INVENTION

In one embodiment, said loop sequences comprise 4, 5, 6 or 7 amino acid acids.

In a further embodiment, said loop sequences comprise three cysteine residues separated by two loop sequences both of which consist of 5 amino acids (such as those listed in Tables 3, 4 and 9).

In a further embodiment, said loop sequences comprise three cysteine residues separated by two loop sequences both of which consist of 6 amino acids (such as those listed in Tables 3 to 10).

In a further embodiment, said loop sequences comprise three cysteine residues separated by two loop sequences one of which consists of 6 amino acids and the other of which consists of 5 amino acids (such as those listed in Table 4).

In a further embodiment, said loop sequences comprise three cysteine residues separated by two loop sequences one of which consists of 6 amino acids and the other of which consists of 4 amino acids (such as those listed in Table 4).

In a further embodiment, said loop sequences comprise three cysteine residues separated by two loop sequences one of which consists of 6 amino acids and the other of which consists of 7 amino acids (such as those listed in Table 8).

In one embodiment, the peptide ligand comprises an amino acid sequence selected from: C_(i)—X₁—C_(ii)—X₂—C_(iii) wherein X₁ and X₂ represent the amino acid residues between the cysteine residues listed in Tables 3 to 10 and C_(i), C_(ii) and C_(iii) represent first, second and third cysteine residues, respectively or a pharmaceutically acceptable salt thereof.

In a further embodiment, the peptide ligand comprises an amino acid sequence selected from one or more of the peptide ligands listed in one or more Tables 3 to 10.

In one embodiment, the molecular scaffold is selected from (1,3,5-tris(bromomethyl)benzene) (TBMB) and the peptide ligand is selected from any one of the peptide ligands listed in Tables 3 to 10.

In one embodiment, the peptide ligand is selected from any one of Compounds 1-308 or a pharmaceutically acceptable salt thereof.

In a further embodiment, the peptide ligand is selected from any one of Compounds 1-286, 289, 292-293 and 296-297 or a pharmaceutically acceptable salt thereof.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the art, such as in the arts of peptide chemistry, cell culture and phage display, nucleic acid chemistry and biochemistry. Standard techniques are used for molecular biology, genetic and biochemical methods (see Sambrook et al., Molecular Cloning: A Laboratory Manual, 3rd ed., 2001, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.; Ausubel et al., Short Protocols in Molecular Biology (1999) 4th ed., John Wiley & Sons, Inc.), which are incorporated herein by reference.

Nomenclature

Numbering

When referring to amino acid residue positions within the peptides of the invention, cysteine residues (C_(i), C_(ii) and C_(iii)) are omitted from the numbering as they are invariant, therefore, the numbering of amino acid residues within the peptides of the invention is referred to as below:

(SEQ ID NO: 1) -C_(i)-M₁-N₂-D₃-W₄-L₅-C_(ii)-S₆-L₇-G₈-W₉-T₁₀-C_(iii)-. For the purpose of this description, all bicyclic peptides are assumed to be cyclised with either TBMB (1,3,5-tris(bromomethyl)benzene) yielding a tri-substituted 1,3,5-trismethylbenzene structure. Cyclisation with TBMB occurs on C_(i), C_(ii), and C_(iii). Molecular Format

N- or C-terminal extensions to the bicycle core sequence are added to the left or right side of the sequence, separated by a hyphen. For example, an N-terminal (β-Ala)-Sar₁₀-Ala tail would be denoted as:

(SEQ ID NO: X) (β-Ala)-Sar₁₀-A-. Inversed Peptide Sequences

In light of the disclosure in Nair et al (2003) J Immunol 170(3), 1362-1373, it is envisaged that the peptide sequences disclosed herein would also find utility in their retro-inverso form. For example, the sequence is reversed (i.e. N-terminus become C-terminus and vice versa) and their stereochemistry is likewise also reversed (i.e. D-amino acids become L-amino acids and vice versa).

Peptide Ligands

A peptide ligand, as referred to herein, refers to a peptide, peptidic or peptidomimetic covalently bound to a molecular scaffold. Typically, such peptides, peptidics or peptidomimetics comprise a peptide having natural or non-natural amino acids, two or more reactive groups (i.e. cysteine residues) which are capable of forming covalent bonds to the scaffold, and a sequence subtended between said reactive groups which is referred to as the loop sequence, since it forms a loop when the peptide, peptidic or peptidomimetic is bound to the scaffold. In the present case, the peptides, peptidics or peptidomimetics comprise at least three cysteine residues (referred to herein as C_(i), C_(ii), and C_(iii)), and form at least two loops on the scaffold.

Advantages of the Peptide Ligands

Certain bicyclic peptides of the present invention have a number of advantageous properties which enable them to be considered as suitable drug-like molecules for injection, inhalation, nasal, ocular, oral or topical administration. Such advantageous properties include:

-   -   Species cross-reactivity. This is a typical requirement for         preclinical pharmacodynamics and pharmacokinetic evaluation;     -   Protease stability. Bicyclic peptide ligands should in most         circumstances demonstrate stability to plasma proteases,         epithelial (“membrane-anchored”) proteases, gastric and         intestinal proteases, lung surface proteases, intracellular         proteases and the like. Protease stability should be maintained         between different species such that a bicyclic peptide lead         candidate can be developed in animal models as well as         administered with confidence to humans;     -   Desirable solubility profile. This is a function of the         proportion of charged and hydrophilic versus hydrophobic         residues and intra/inter-molecular H-bonding, which is important         for formulation and absorption purposes;     -   An optimal plasma half-life in the circulation. Depending upon         the clinical indication and treatment regimen, it may be         required to develop a bicyclic peptide with short or prolonged         in vivo exposure times for the management of either chronic or         acute disease states. The optimal exposure time will be governed         by the requirement for sustained exposure (for maximal         therapeutic efficiency) versus the requirement for short         exposure times to minimise toxicological effects arising from         sustained exposure to the agent; and     -   Selectivity. Certain peptide ligands of the invention         demonstrate good selectivity over other Eph receptor tyrosine         kinases, such as EphA1, EphA3, EphA4, EphA6, EphA7 and EphB1 and         factor XIIA, carbonic anhydrase 9 and CD38 (selectivity data for         selected peptide ligands of the invention may be seen in Table         12). It should also be noted that selected peptide ligands of         the invention exhibit cross reactivity with other species (eg         mouse) to permit testing in animal models (Tables 3, 5, 7, 8 and         11).

Pharmaceutically Acceptable Salts

It will be appreciated that salt forms are within the scope of this invention, and references to peptide ligands include the salt forms of said ligands.

The salts of the present invention can be synthesized from the parent compound that contains a basic or acidic moiety by conventional chemical methods such as methods described in Pharmaceutical Salts: Properties, Selection, and Use, P. Heinrich Stahl (Editor), Camille G. Wermuth (Editor), ISBN: 3-90639-026-8, Hardcover, 388 pages, August 2002. Generally, such salts can be prepared by reacting the free acid or base forms of these compounds with the appropriate base or acid in water or in an organic solvent, or in a mixture of the two.

Acid addition salts (mono- or di-salts) may be formed with a wide variety of acids, both inorganic and organic. Examples of acid addition salts include mono- or di-salts formed with an acid selected from the group consisting of acetic, 2,2-dichloroacetic, adipic, alginic, ascorbic (e.g. L-ascorbic), L-aspartic, benzenesulfonic, benzoic, 4-acetamidobenzoic, butanoic, (+) camphoric, camphor-sulfonic, (+)-(1S)-camphor-10-sulfonic, capric, caproic, caprylic, cinnamic, citric, cyclamic, dodecylsulfuric, ethane-1,2-disulfonic, ethanesulfonic, 2-hydroxyethanesulfonic, formic, fumaric, galactaric, gentisic, glucoheptonic, D-gluconic, glucuronic (e.g. D-glucuronic), glutamic (e.g. L-glutamic), α-oxoglutaric, glycolic, hippuric, hydrohalic acids (e.g. hydrobromic, hydrochloric, hydriodic), isethionic, lactic (e.g. (+)-L-lactic, (±)-DL-lactic), lactobionic, maleic, malic, (−)-L-malic, malonic, (±)-DL-mandelic, methanesulfonic, naphthalene-2-sulfonic, naphthalene-1,5-disulfonic, 1-hydroxy-2-naphthoic, nicotinic, nitric, oleic, orotic, oxalic, palmitic, pamoic, phosphoric, propionic, pyruvic, L-pyroglutamic, salicylic, 4-amino-salicylic, sebacic, stearic, succinic, sulfuric, tannic, (+)-L-tartaric, thiocyanic, p-toluenesulfonic, undecylenic and valeric acids, as well as acylated amino acids and cation exchange resins.

One particular group of salts consists of salts formed from acetic, hydrochloric, hydriodic, phosphoric, nitric, sulfuric, citric, lactic, succinic, maleic, malic, isethionic, fumaric, benzenesulfonic, toluenesulfonic, sulfuric, methanesulfonic (mesylate), ethanesulfonic, naphthalenesulfonic, valeric, propanoic, butanoic, malonic, glucuronic and lactobionic acids. One particular salt is the hydrochloride salt. Another particular salt is the acetate salt.

If the compound is anionic, or has a functional group which may be anionic (e.g., —COOH may be —COO⁻), then a salt may be formed with an organic or inorganic base, generating a suitable cation. Examples of suitable inorganic cations include, but are not limited to, alkali metal ions such as Li⁺, Na⁺ and K⁺, alkaline earth metal cations such as Ca²⁺ and Mg²⁺, and other cations such as Al³⁺ or Zn⁺. Examples of suitable organic cations include, but are not limited to, ammonium ion (i.e., NH₄ ⁺) and substituted ammonium ions (e.g., NH₃R⁺, NH₂R₂ ⁺, NHR₃ ⁺, NR₄ ⁺). Examples of some suitable substituted ammonium ions are those derived from: methylamine, ethylamine, diethylamine, propylamine, dicyclohexylamine, triethylamine, butylamine, ethylenediamine, ethanolamine, diethanolamine, piperazine, benzylamine, phenylbenzylamine, choline, meglumine, and tromethamine, as well as amino acids, such as lysine and arginine. An example of a common quaternary ammonium ion is N(CH₃)₄ ⁺.

Where the peptides of the invention contain an amine function, these may form quaternary ammonium salts, for example by reaction with an alkylating agent according to methods well known to the skilled person. Such quaternary ammonium compounds are within the scope of the peptides of the invention.

Modified Derivatives

It will be appreciated that modified derivatives of the peptide ligands as defined herein are within the scope of the present invention. Examples of such suitable modified derivatives include one or more modifications selected from: N-terminal and/or C-terminal modifications;

replacement of one or more amino acid residues with one or more non-natural amino acid residues (such as replacement of one or more polar amino acid residues with one or more isosteric or isoelectronic amino acids; replacement of one or more non-polar amino acid residues with other non-natural isosteric or isoelectronic amino acids); addition of a spacer group; replacement of one or more oxidation sensitive amino acid residues with one or more oxidation resistant amino acid residues; replacement of one or more amino acid residues with one or more replacement amino acids, such as an alanine, replacement of one or more L-amino acid residues with one or more D-amino acid residues; N-alkylation of one or more amide bonds within the bicyclic peptide ligand; replacement of one or more peptide bonds with a surrogate bond; peptide backbone length modification; substitution of the hydrogen on the alpha-carbon of one or more amino acid residues with another chemical group, modification of amino acids such as cysteine, lysine, glutamate/aspartate and tyrosine with suitable amine, thiol, carboxylic acid and phenol-reactive reagents so as to functionalise said amino acids, and introduction or replacement of amino acids that introduce orthogonal reactivities that are suitable for functionalisation, for example azide or alkyne-group bearing amino acids that allow functionalisation with alkyne or azide-bearing moieties, respectively.

In one embodiment, the modified derivative comprises an N-terminal and/or C-terminal modification. In a further embodiment, wherein the modified derivative comprises an N-terminal modification using suitable amino-reactive chemistry, and/or C-terminal modification using suitable carboxy-reactive chemistry. In a further embodiment, said N-terminal or C-terminal modification comprises addition of an effector group, including but not limited to a cytotoxic agent, a radiochelator or a chromophore.

In a further embodiment, the modified derivative comprises an N-terminal modification. In a further embodiment, the N-terminal modification comprises an N-terminal acetyl group. In this embodiment, the N-terminal residue is capped with acetic anhydride or other appropriate reagents during peptide synthesis leading to a molecule which is N-terminally acetylated. This embodiment provides the advantage of removing a potential recognition point for aminopeptidases and avoids the potential for degradation of the bicyclic peptide.

In an alternative embodiment, the N-terminal modification comprises the addition of a molecular spacer group which facilitates the conjugation of effector groups and retention of potency of the bicyclic peptide to its target.

In a further embodiment, the modified derivative comprises a C-terminal modification. In a further embodiment, the C-terminal modification comprises an amide group. In this embodiment, the C-terminal residue is synthesized as an amide during peptide synthesis leading to a molecule which is C-terminally amidated. This embodiment provides the advantage of removing a potential recognition point for carboxypeptidase and reduces the potential for proteolytic degradation of the bicyclic peptide.

In one embodiment, the modified derivative comprises replacement of one or more amino acid residues with one or more non-natural amino acid residues. In this embodiment, non-natural amino acids may be selected having isosteric/isoelectronic side chains which are neither recognised by degradative proteases nor have any adverse effect upon target potency.

Alternatively, non-natural amino acids may be used having constrained amino acid side chains, such that proteolytic hydrolysis of the nearby peptide bond is conformationally and sterically impeded. In particular, these concern proline analogues, bulky sidechains, Cα-disubstituted derivatives (for example, aminoisobutyric acid, Aib), and cyclo amino acids, a simple derivative being amino-cyclopropylcarboxylic acid.

In one embodiment, the modified derivative comprises the addition of a spacer group. In a further embodiment, the modified derivative comprises the addition of a spacer group to the N-terminal cysteine (C_(i)) and/or the C-terminal cysteine (C_(iii)).

In one embodiment, the modified derivative comprises replacement of one or more oxidation sensitive amino acid residues with one or more oxidation resistant amino acid residues. In a further embodiment, the modified derivative comprises replacement of a tryptophan residue with a naphthylalanine or alanine residue. This embodiment provides the advantage of improving the pharmaceutical stability profile of the resultant bicyclic peptide ligand.

In one embodiment, the modified derivative comprises replacement of one or more charged amino acid residues with one or more hydrophobic amino acid residues. In an alternative embodiment, the modified derivative comprises replacement of one or more hydrophobic amino acid residues with one or more charged amino acid residues. The correct balance of charged versus hydrophobic amino acid residues is an important characteristic of the bicyclic peptide ligands. For example, hydrophobic amino acid residues influence the degree of plasma protein binding and thus the concentration of the free available fraction in plasma, while charged amino acid residues (in particular arginine) may influence the interaction of the peptide with the phospholipid membranes on cell surfaces. The two in combination may influence half-life, volume of distribution and exposure of the peptide drug, and can be tailored according to the clinical endpoint. In addition, the correct combination and number of charged versus hydrophobic amino acid residues may reduce irritation at the injection site (if the peptide drug has been administered subcutaneously).

In one embodiment, the modified derivative comprises replacement of one or more L-amino acid residues with one or more D-amino acid residues. This embodiment is believed to increase proteolytic stability by steric hindrance and by a propensity of D-amino acids to stabilise β-turn conformations (Tugyi et al (2005) PNAS, 102(2), 413-418).

In one embodiment, the modified derivative comprises removal of any amino acid residues and substitution with alanines, such as D-alanines. This embodiment provides the advantage of identifying key binding residues and removing potential proteolytic attack site(s). The results of such alanine, such as D-alanine substitutions (also known as alanine scanning) may be seen in Table 9.

It should be noted that each of the above mentioned modifications serve to deliberately improve the potency or stability of the peptide. Further potency improvements based on modifications may be achieved through the following mechanisms:

-   -   Incorporating hydrophobic moieties that exploit the hydrophobic         effect and lead to lower off rates, such that higher affinities         are achieved;     -   Incorporating charged groups that exploit long-range ionic         interactions, leading to faster on rates and to higher         affinities (see for example Schreiber et al, Rapid,         electrostatically assisted association of proteins (1996),         Nature Struct. Biol. 3, 427-31); and     -   Incorporating additional constraint into the peptide, by for         example constraining side chains of amino acids correctly such         that loss in entropy is minimal upon target binding,         constraining the torsional angles of the backbone such that loss         in entropy is minimal upon target binding and introducing         additional cyclisations in the molecule for identical reasons.

(for reviews see Gentilucci et al, Curr. Pharmaceutical Design, (2010), 16, 3185-203, and

Nestor et al, Curr. Medicinal Chem (2009), 16, 4399-418).

Isotopic Variations

The present invention includes all pharmaceutically acceptable (radio)isotope-labeled peptide ligands of the invention, wherein one or more atoms are replaced by atoms having the same atomic number, but an atomic mass or mass number different from the atomic mass or mass number usually found in nature, and peptide ligands of the invention, wherein metal chelating groups are attached (termed “effector”) that are capable of holding relevant (radio)isotopes, and peptide ligands of the invention, wherein certain functional groups are covalently replaced with relevant (radio)isotopes or isotopically labelled functional groups.

Examples of isotopes suitable for inclusion in the peptide ligands of the invention comprise isotopes of hydrogen, such as ²H (D) and ³H (T), carbon, such as ¹¹C, ¹³C and ¹⁴C, chlorine, such as ³⁶Cl, fluorine, such as ¹⁸F, iodine, such as ¹²³I, ¹²⁵I and ¹³¹I, nitrogen, such as ¹³N and ¹⁵N, oxygen, such as ¹⁵O, ¹⁷O and ¹⁸O, phosphorus, such as ³²P, sulfur, such as ³⁵S, copper, such as ⁶⁴Cu, gallium, such as ⁶⁷Ga or ⁶⁸Ga, yttrium, such as ⁹⁰Y and lutetium, such as ¹⁷⁷Lu, and Bismuth, such as ²¹³Bi.

Certain isotopically-labelled peptide ligands of the invention, for example, those incorporating a radioactive isotope, are useful in drug and/or substrate tissue distribution studies, and to clinically assess the presence and/or absence of the EphA2 target on diseased tissues. The peptide ligands of the invention can further have valuable diagnostic properties in that they can be used for detecting or identifying the formation of a complex between a labelled compound and other molecules, peptides, proteins, enzymes or receptors. The detecting or identifying methods can use compounds that are labelled with labelling agents such as radioisotopes, enzymes, fluorescent substances, luminous substances (for example, luminol, luminol derivatives, luciferin, aequorin and luciferase), etc. The radioactive isotopes tritium, i.e. ³H (T), and carbon-14, i.e. ¹⁴C, are particularly useful for this purpose in view of their ease of incorporation and ready means of detection.

Substitution with heavier isotopes such as deuterium, i.e. ²H (D), may afford certain therapeutic advantages resulting from greater metabolic stability, for example, increased in vivo half-life or reduced dosage requirements, and hence may be preferred in some circumstances.

Substitution with positron emitting isotopes, such as ¹¹C, ¹⁸F, ¹⁵O and ¹³N, can be useful in Positron Emission Topography (PET) studies for examining target occupancy.

Isotopically-labeled compounds of peptide ligands of the invention can generally be prepared by conventional techniques known to those skilled in the art or by processes analogous to those described in the accompanying Examples using an appropriate isotopically-labeled reagent in place of the non-labeled reagent previously employed.

Aromatic Molecular Scaffold

References herein to the term “aromatic molecular scaffold” refer to any molecular scaffold as defined herein which contains an aromatic (i.e. unsaturated) carbocyclic or heterocyclic ring system.

It will be appreciated that the aromatic molecular scaffold may comprise an aromatic moiety. Examples of suitable aromatic moieties within the aromatic scaffold include biphenylene, terphenylene, naphthalene or anthracene.

It will also be appreciated that the aromatic molecular scaffold may comprise a heteroaromatic moiety. Examples of suitable heteroaromatic moieties within the aromatic scaffold include pyridine, pyrimidine, pyrrole, furan and thiopene.

It will also be appreciated that the aromatic molecular scaffold may comprise a halomethylarene moiety, such as a bis(bromomethyl)benzene, a tris(bromomethyl)benzene, a tetra(bromomethyl)benzene or derivatives thereof.

Non-limiting examples of aromatic molecular scaffolds include: bis-, tris-, or tetra(halomethyl)benzene; bis-, tris-, or tetra(halomethyl)pyridine; bis-, tris-, or tetra(halomethyl)pyridazine; bis-, tris-, or tetra(halomethyl)pyrimidine; bis-, tris-, or tetra(halomethyl)pyrazine; bis-, tris-, or tetra(halomethyl)-1,2,3-triazine; bis-, tris-, or tetra-halomethyl)-1,2,4-triazine; bis-, tris-, or tetra(halomethyl)pyrrole, -furan, -thiophene; bis-, tris-, or tetra(halomethyl)imidazole, -oxazole, -thiazol; bis-, tris-, or tetra(halomethyl)-3H-pyrazole, -isooxazole, -isothiazol; bis-, tris-, or tetra(halomethyl)biphenylene; bis-, tris-, or tetra(halomethyl)terphenylene; 1,8-bis(halomethyl)naphthalene; bis-, tris-, or tetra(halomethyl)anthracene; and bis-, tris-, or tetra(2-halomethylphenyl)methane.

More specific examples of aromatic molecular scaffolds include: 1,2-bis(halomethyl)benzene; 3,4-bis(halomethyl)pyridine; 3,4-bis(halomethyl)pyridazine; 4,5-bis(halomethyl)pyrimidine; 4,5-bis(halomethyl)pyrazine; 4,5-bis(halomethyl)-1,2,3-triazine; 5,6-bis(halomethyl)-1,2,4-triazine; 3,4-bis(halomethyl)pyrrole, -furan, -thiophene and other regioisomers; 4,5-bis(halomethyl)imidazole, -oxazole, -thiazol; 4,5-bis(halomethyl)-3H-pyrazole, -isooxazole, -isothiazol; 2,2′-bis(halomethyl)biphenylene; 2,2″-bis(halomethyl)terphenylene; 1,8-bis(halomethyl)naphthalene; 1,10-bis(halomethyl)anthracene; bis(2-halomethylphenyl)methane; 1,2,3-tris(halomethyl)benzene; 2,3,4-tris(halomethyl)pyridine; 2,3,4-tris(halomethyl)pyridazine; 3,4,5-tris(halomethyl)pyrimidine; 4,5,6-tris(halomethyl)-1,2,3-triazine; 2,3,4-tris(halomethyl)pyrrole, -furan, -thiophene; 2,4,5-bis(halomethyl)imidazole, -oxazole, -thiazol; 3,4,5-bis(halomethyl)-1H-pyrazole, -isooxazole, -isothiazol; 2,4,2′-tris(halomethyl)biphenylene; 2,3′, 2″-tris(halomethyl)terphenylene; 1,3,8-tris(halomethyl)naphthalene; 1,3,10-tris(halomethyl)anthracene; bis(2-halomethylphenyl)methane; 1,2,4,5-tetra(halomethyl)benzene; 1,2,4,5-tetra(halomethyl)pyridine; 2,4,5,6-tetra(halomethyl)pyrimidine; 2,3,4,5-tetra(halomethyl)pyrrole, -furan, -thiophene; 2,2′,6,6′-tetra(halomethyl)biphenylene; 2,2″,6,6″-tetra(halomethyl) terphenylene; 2,3,5,6-tetra(halomethyl)naphthalene and 2,3,7,8-tetra(halomethyl)anthracene; and bis(2,4-bis(halomethyl)phenyl)methane.

As noted in the foregoing documents, the molecular scaffold may be a small molecule, such as a small organic molecule.

In one embodiment the molecular scaffold may be a macromolecule. In one embodiment the molecular scaffold is a macromolecule composed of amino acids, nucleotides or carbohydrates.

In one embodiment the molecular scaffold comprises reactive groups that are capable of reacting with functional group(s) of the polypeptide to form covalent bonds.

The molecular scaffold may comprise chemical groups which form the linkage with a peptide, such as amines, thiols, alcohols, ketones, aldehydes, nitriles, carboxylic acids, esters, alkenes, alkynes, azides, anhydrides, succinimides, maleimides, alkyl halides and acyl halides.

In one embodiment, the molecular scaffold may comprise or may consist of tris(bromomethyl)benzene, especially 1,3,5-tris(bromomethyl)benzene (‘TBMB’), or a derivative thereof.

In one embodiment, the molecular scaffold is 2,4,6-tris(bromomethyl)mesitylene. This molecule is similar to 1,3,5-tris(bromomethyl)benzene but contains three additional methyl groups attached to the benzene ring. This has the advantage that the additional methyl groups may form further contacts with the polypeptide and hence add additional structural constraint.

The molecular scaffold of the invention contains chemical groups that allow functional groups of the polypeptide of the encoded library of the invention to form covalent links with the molecular scaffold. Said chemical groups are selected from a wide range of functionalities including amines, thiols, alcohols, ketones, aldehydes, nitriles, carboxylic acids, esters, alkenes, alkynes, anhydrides, succinimides, maleimides, azides, alkyl halides and acyl halides.

Scaffold reactive groups that could be used on the molecular scaffold to react with thiol groups of cysteines are alkyl halides (or also named halogenoalkanes or haloalkanes).

Examples include bromomethylbenzene (the scaffold reactive group exemplified by TBMB) or iodoacetamide. Other scaffold reactive groups that are used to selectively couple compounds to cysteines in proteins are maleimides, αβ unsaturated carbonyl containing compounds and α-halomethylcarbonyl containing compounds. Examples of maleimides which may be used as molecular scaffolds in the invention include: tris-(2-maleimidoethyl)amine, tris-(2-maleimidoethyl)benzene, tris-(maleimido)benzene. An example of an α-halomethylcarbonyl containing compound is N,N′,N″-(benzene-1,3,5-triyl)tris(2-bromoacetamide). Selenocysteine is also a natural amino acid which has a similar reactivity to cysteine and can be used for the same reactions. Thus, wherever cysteine is mentioned, it is typically acceptable to substitute selenocysteine unless the context suggests otherwise.

Effector and Functional Groups

According to a further aspect of the invention, there is provided a drug conjugate comprising a peptide ligand as defined herein conjugated to one or more effector and/or functional groups.

Effector and/or functional groups can be attached, for example, to the N and/or C termini of the polypeptide, to an amino acid within the polypeptide, or to the molecular scaffold.

Appropriate effector groups include antibodies and parts or fragments thereof. For instance, an effector group can include an antibody light chain constant region (CL), an antibody CH1 heavy chain domain, an antibody CH2 heavy chain domain, an antibody CH3 heavy chain domain, or any combination thereof, in addition to the one or more constant region domains. An effector group may also comprise a hinge region of an antibody (such a region normally being found between the CH1 and CH2 domains of an IgG molecule).

In a further embodiment of this aspect of the invention, an effector group according to the present invention is an Fc region of an IgG molecule. Advantageously, a peptide ligand-effector group according to the present invention comprises or consists of a peptide ligand Fc fusion having a tβ half-life of a day or more, two days or more, 3 days or more, 4 days or more, 5 days or more, 6 days or more or 7 days or more. Most advantageously, the peptide ligand according to the present invention comprises or consists of a peptide ligand Fc fusion having a tβ half-life of a day or more.

Functional groups include, in general, binding groups, drugs, reactive groups for the attachment of other entities, functional groups which aid uptake of the macrocyclic peptides into cells, and the like.

The ability of peptides to penetrate into cells will allow peptides against intracellular targets to be effective. Targets that can be accessed by peptides with the ability to penetrate into cells include transcription factors, intracellular signalling molecules such as tyrosine kinases and molecules involved in the apoptotic pathway. Functional groups which enable the penetration of cells include peptides or chemical groups which have been added either to the peptide or the molecular scaffold. Peptides such as those derived from such as VP22, HIV-Tat, a homeobox protein of Drosophila (Antennapedia), e.g. as described in Chen and Harrison, Biochemical Society Transactions (2007) Volume 35, part 4, p821; Gupta et al. in Advanced Drug Discovery Reviews (2004) Volume 57 9637. Examples of short peptides which have been shown to be efficient at translocation through plasma membranes include the 16 amino acid penetratin peptide from Drosophila Antennapedia protein (Derossi et al (1994) J Biol. Chem. Volume 269 p10444), the 18 amino acid ‘model amphipathic peptide’ (Oehlke et al (1998) Biochim Biophys Acts Volume 1414 p127) and arginine rich regions of the HIV TAT protein. Non peptidic approaches include the use of small molecule mimics or SMOCs that can be easily attached to biomolecules (Okuyama et al (2007) Nature Methods Volume 4 p153). Other chemical strategies to add guanidinium groups to molecules also enhance cell penetration (Elson-Scwab et al (2007) J Biol Chem Volume 282 p13585). Small molecular weight molecules such as steroids may be added to the molecular scaffold to enhance uptake into cells.

One class of functional groups which may be attached to peptide ligands includes antibodies and binding fragments thereof, such as Fab, Fv or single domain fragments. In particular, antibodies which bind to proteins capable of increasing the half-life of the peptide ligand in vivo may be used.

In one embodiment, a peptide ligand-effector group according to the invention has a tβ half-life selected from the group consisting of: 12 hours or more, 24 hours or more, 2 days or more, 3 days or more, 4 days or more, 5 days or more, 6 days or more, 7 days or more, 8 days or more, 9 days or more, 10 days or more, 11 days or more, 12 days or more, 13 days or more, 14 days or more, 15 days or more or 20 days or more. Advantageously a peptide ligand-effector group or composition according to the invention will have a tβ half life in the range 12 to 60 hours. In a further embodiment, it will have a tβ half-life of a day or more. In a further embodiment still, it will be in the range 12 to 26 hours.

In one particular embodiment of the invention, the functional group is selected from a metal chelator, which is suitable for complexing metal radioisotopes of medicinal relevance.

Possible effector groups also include enzymes, for instance such as carboxypeptidase G2 for use in enzyme/prodrug therapy, where the peptide ligand replaces antibodies in ADEPT.

In one particular embodiment of the invention, the functional group is selected from a drug, such as a cytotoxic agent for cancer therapy. Suitable examples include: alkylating agents such as cisplatin and carboplatin, as well as oxaliplatin, mechlorethamine, cyclophosphamide, chlorambucil, ifosfamide; Anti-metabolites including purine analogs azathioprine and mercaptopurine or pyrimidine analogs; plant alkaloids and terpenoids including vinca alkaloids such as Vincristine, Vinblastine, Vinorelbine and Vindesine; Podophyllotoxin and its derivatives etoposide and teniposide; Taxanes, including paclitaxel, originally known as Taxol; topoisomerase inhibitors including camptothecins: irinotecan and topotecan, and type II inhibitors including amsacrine, etoposide, etoposide phosphate, and teniposide. Further agents can include antitumour antibiotics which include the immunosuppressant dactinomycin (which is used in kidney transplantations), doxorubicin, epirubicin, bleomycin, calicheamycins, and others.

In one further particular embodiment of the invention, the cytotoxic agent is selected from maytansinoids (such as DM1) or monomethyl auristatins (such as MMAE).

DM1 is a cytotoxic agent which is a thiol-containing derivative of maytansine and has the following structure:

Monomethyl auristatin E (MMAE) is a synthetic antineoplastic agent and has the following structure:

In one yet further particular embodiment of the invention, the cytotoxic agent is selected from maytansinoids (such as DM1). Data is presented herein in Table 11 which demonstrates the effects of peptide ligands conjugated to toxins containing DM1.

In one embodiment, the cytotoxic agent is linked to the bicyclic peptide by a cleavable bond, such as a disulphide bond or a protease sensitive bond. In a further embodiment, the groups adjacent to the disulphide bond are modified to control the hindrance of the disulphide bond, and by this the rate of cleavage and concomitant release of cytotoxic agent.

Published work established the potential for modifying the susceptibility of the disulphide bond to reduction by introducing steric hindrance on either side of the disulphide bond (Kellogg et al (2011) Bioconjugate Chemistry, 22, 717). A greater degree of steric hindrance reduces the rate of reduction by intracellular glutathione and also extracellular (systemic) reducing agents, consequentially reducing the ease by which toxin is released, both inside and outside the cell. Thus, selection of the optimum in disulphide stability in the circulation (which minimises undesirable side effects of the toxin) versus efficient release in the intracellular milieu (which maximises the therapeutic effect) can be achieved by careful selection of the degree of hindrance on either side of the disulphide bond.

The hindrance on either side of the disulphide bond is modulated through introducing one or more methyl groups on either the targeting entity (here, the bicyclic peptide) or toxin side of the molecular construct.

In one embodiment, the cytotoxic agent and linker is selected from any combinations of those described in WO 2016/067035 (the cytotoxic agents and linkers thereof are herein incorporated by reference).

In one embodiment, the cytotoxic agent is DM1 and the drug conjugate comprises a compound of formula (A):

wherein said bicycle is selected from any one of Compound 72 as defined herein.

In an alternative embodiment, the cytotoxic agent is DM1 and the drug conjugate comprises a compound of formula (B):

wherein said bicycle is selected from any one of Compounds 72, 226, 227 and 303 as defined herein.

In one embodiment, the cytotoxic agent is DM1 and the drug conjugate comprises a compound of formula (A), wherein said bicycle is selected from Compound 72 as defined herein. This BDC is known herein as BDC-1. Data is presented herein which demonstrates excellent competition binding for BDC-1 in the EphA2 competition binding assay as shown in Table 11.

In an alternative embodiment, the cytotoxic agent is DM1 and the drug conjugate comprises a compound of formula (B), wherein said bicycle is selected from Compound 72 as defined herein. This BDC is known herein as BDC-2. Data is presented herein which demonstrates excellent competition binding for BDC-2 in the EphA2 competition binding assay as shown in Table 11.

In an alternative embodiment, the cytotoxic agent is DM1 and the drug conjugate comprises a compound of formula (B), wherein said bicycle is selected from Compound 226 as defined herein. This BDC is known herein as BDC-3. Data is presented herein which demonstrates excellent competition binding for BDC-3 in the EphA2 competition binding assay as shown in Table 11.

In an alternative embodiment, the cytotoxic agent is DM1 and the drug conjugate comprises a compound of formula (B), wherein said bicycle is selected from Compound 227 as defined herein. This BDC is known herein as BDC-4. Data is presented herein which demonstrates excellent competition binding for BDC-4 in the EphA2 competition binding assay as shown in Table 11.

In an alternative embodiment, the cytotoxic agent is DM1 and the drug conjugate comprises a compound of formula (B), wherein said bicycle is selected from Compound 303 as defined herein. This BDC is known herein as BDC-5. Data is presented herein which demonstrates excellent competition binding for BDC-5 in the EphA2 competition binding assay as shown in Table 11.

In one embodiment, the drug conjugate is selected from BDC-1 to BDC-5. In a further embodiment, the drug conjugate is selected from BDC-1 to BDC-4.

Synthesis

The peptides of the present invention may be manufactured synthetically by standard techniques followed by reaction with a molecular scaffold in vitro. When this is performed, standard chemistry may be used. This enables the rapid large scale preparation of soluble material for further downstream experiments or validation. Such methods could be accomplished using conventional chemistry such as that disclosed in Timmerman et al (supra).

Thus, the invention also relates to manufacture of polypeptides or conjugates selected as set out herein, wherein the manufacture comprises optional further steps as explained below. In one embodiment, these steps are carried out on the end product polypeptide/conjugate made by chemical synthesis.

Optionally amino acid residues in the polypeptide of interest may be substituted when manufacturing a conjugate or complex.

Peptides can also be extended, to incorporate for example another loop and therefore introduce multiple specificities.

To extend the peptide, it may simply be extended chemically at its N-terminus or C-terminus or within the loops using orthogonally protected lysines (and analogues) using standard solid phase or solution phase chemistry. Standard (bio)conjugation techniques may be used to introduce an activated or activatable N- or C-terminus. Alternatively additions may be made by fragment condensation or native chemical ligation e.g. as described in (Dawson et al. 1994. Synthesis of Proteins by Native Chemical Ligation. Science 266:776-779), or by enzymes, for example using subtiligase as described in (Chang et al Proc Natl Acad Sci USA. 1994 Dec. 20; 91(26):12544-8 or in Hikari et al Bioorganic & Medicinal Chemistry Letters Volume 18, Issue 22, 15 Nov. 2008, Pages 6000-6003).

Alternatively, the peptides may be extended or modified by further conjugation through disulphide bonds. This has the additional advantage of allowing the first and second peptide to dissociate from each other once within the reducing environment of the cell. In this case, the molecular scaffold (e.g. TBMB) could be added during the chemical synthesis of the first peptide so as to react with the three cysteine groups; a further cysteine or thiol could then be appended to the N or C-terminus of the first peptide, so that this cysteine or thiol only reacted with a free cysteine or thiol of the second peptide, forming a disulfide-linked bicyclic peptide-peptide conjugate.

Similar techniques apply equally to the synthesis/coupling of two bicyclic and bispecific macrocycles, potentially creating a tetraspecific molecule.

Furthermore, addition of other functional groups or effector groups may be accomplished in the same manner, using appropriate chemistry, coupling at the N- or C-termini or via side chains. In one embodiment, the coupling is conducted in such a manner that it does not block the activity of either entity.

Pharmaceutical Compositions

According to a further aspect of the invention, there is provided a pharmaceutical composition comprising a peptide ligand or a drug conjugate as defined herein in combination with one or more pharmaceutically acceptable excipients.

Generally, the present peptide ligands will be utilised in purified form together with pharmacologically appropriate excipients or carriers. Typically, these excipients or carriers include aqueous or alcoholic/aqueous solutions, emulsions or suspensions, including saline and/or buffered media. Parenteral vehicles include sodium chloride solution, Ringer's dextrose, dextrose and sodium chloride and lactated Ringer's. Suitable physiologically-acceptable adjuvants, if necessary to keep a polypeptide complex in suspension, may be chosen from thickeners such as carboxymethylcellulose, polyvinylpyrrolidone, gelatin and alginates.

Intravenous vehicles include fluid and nutrient replenishers and electrolyte replenishers, such as those based on Ringer's dextrose. Preservatives and other additives, such as antimicrobials, antioxidants, chelating agents and inert gases, may also be present (Mack (1982) Remington's Pharmaceutical Sciences, 16th Edition).

The peptide ligands of the present invention may be used as separately administered compositions or in conjunction with other agents. These can include antibodies, antibody fragments and various immunotherapeutic drugs, such as cylcosporine, methotrexate, adriamycin or cisplatinum and immunotoxins. Pharmaceutical compositions can include “cocktails” of various cytotoxic or other agents in conjunction with the protein ligands of the present invention, or even combinations of selected polypeptides according to the present invention having different specificities, such as polypeptides selected using different target ligands, whether or not they are pooled prior to administration.

The route of administration of pharmaceutical compositions according to the invention may be any of those commonly known to those of ordinary skill in the art. For therapy, the peptide ligands of the invention can be administered to any patient in accordance with standard techniques. The administration can be by any appropriate mode, including parenterally, intravenously, intramuscularly, intraperitoneally, transdermally, via the pulmonary route, or also, appropriately, by direct infusion with a catheter. Preferably, the pharmaceutical compositions according to the invention will be administered by inhalation. The dosage and frequency of administration will depend on the age, sex and condition of the patient, concurrent administration of other drugs, counterindications and other parameters to be taken into account by the clinician.

The peptide ligands of this invention can be lyophilised for storage and reconstituted in a suitable carrier prior to use. This technique has been shown to be effective and art-known lyophilisation and reconstitution techniques can be employed. It will be appreciated by those skilled in the art that lyophilisation and reconstitution can lead to varying degrees of activity loss and that levels may have to be adjusted upward to compensate.

The compositions containing the present peptide ligands or a cocktail thereof can be administered for prophylactic and/or therapeutic treatments. In certain therapeutic applications, an adequate amount to accomplish at least partial inhibition, suppression, modulation, killing, or some other measurable parameter, of a population of selected cells is defined as a “therapeutically-effective dose”. Amounts needed to achieve this dosage will depend upon the severity of the disease and the general state of the patient's own immune system, but generally range from 0.005 to 5.0 mg of selected peptide ligand per kilogram of body weight, with doses of 0.05 to 2.0 mg/kg/dose being more commonly used. For prophylactic applications, compositions containing the present peptide ligands or cocktails thereof may also be administered in similar or slightly lower dosages.

A composition containing a peptide ligand according to the present invention may be utilised in prophylactic and therapeutic settings to aid in the alteration, inactivation, killing or removal of a select target cell population in a mammal. In addition, the peptide ligands described herein may be used extracorporeally or in vitro selectively to kill, deplete or otherwise effectively remove a target cell population from a heterogeneous collection of cells. Blood from a mammal may be combined extracorporeally with the selected peptide ligands whereby the undesired cells are killed or otherwise removed from the blood for return to the mammal in accordance with standard techniques.

Therapeutic Uses

The bicyclic peptides of the invention have specific utility as EphA2 binding agents.

Eph receptor tyrosine kinases (Ephs) belong to a large group of receptor tyrosine kinases (RTKs), kinases that phosphorylate proteins on tyrosine residues. Ephs and their membrane bound ephrin ligands (ephrins) control cell positioning and tissue organization (Poliakov et al. (2004) Dev Cell 7, 465-80). Functional and biochemical Eph responses occur at higher ligand oligomerization states (Stein et al. (1998) Genes Dev 12, 667-678).

Among other patterning functions, various Ephs and ephrins have been shown to play a role in vascular development. Knockout of EphB4 and ephrin-B2 results in a lack of the ability to remodel capillary beds into blood vessels (Poliakov et al., supra) and embryonic lethality. Persistent expression of some Eph receptors and ephrins has also been observed in newly-formed, adult micro-vessels (Brantley-Sieders et al. (2004) Curr Pharm Des 10, 3431-42; Adams (2003) J Anat 202, 105-12).

The de-regulated re-emergence of some ephrins and their receptors in adults also has been observed to contribute to tumor invasion, metastasis and neo-angiogenesis (Nakamoto et al.

(2002) Microsc Res Tech 59, 58-67; Brantley-Sieders et al., supra). Furthermore, some Eph family members have been found to be over-expressed on tumor cells from a variety of human tumors (Brantley-Sieders et al., supra); Marme (2002) Ann Hematol 81 Suppl 2, S66; Booth et al. (2002) Nat Med 8, 1360-1).

EPH receptor A2 (ephrin type-A receptor 2) is a protein that in humans is encoded by the EPHA2 gene.

EphA2 is upregulated in multiple cancers in man, often correlating with disease progression, metastasis and poor prognosis e.g.: breast (Zelinski et al (2001) Cancer Res. 61, 2301-2306; Zhuang et al (2010) Cancer Res. 70, 299-308; Brantley-Sieders et al (2011) PLoS One 6, e24426), lung (Brannan et al (2009) Cancer Prey Res (Phila) 2, 1039-1049; Kinch et al (2003) Clin Cancer Res. 9, 613-618; Guo et al (2013) J Thorac Oncol. 8, 301-308), gastric (Nakamura et al (2005) Cancer Sci. 96, 42-47; Yuan et al (2009) Dig Dis Sci 54, 2410-2417), pancreatic (Mudali et al (2006) Clin Exp Metastasis 23, 357-365), prostate (Walker-Daniels et al (1999) Prostate 41, 275-280), liver (Yang et al (2009) Hepatol Res. 39, 1169-1177) and glioblastoma (Wykosky et al (2005) Mol Cancer Res. 3, 541-551; Li et al (2010) Tumour Biol. 31, 477-488).

The full role of EphA2 in cancer progression is still not defined although there is evidence for interaction at numerous stages of cancer progression including tumour cell growth, survival, invasion and angiogenesis. Downregulation of EphA2 expression suppresses tumour cancer cell propagation (Binda et al (2012) Cancer Cell 22, 765-780), whilst EphA2 blockade inhibits VEGF induced cell migration (Hess et al (2001) Cancer Res. 61, 3250-3255), sprouting and angiogenesis (Cheng et al (2002) Mol Cancer Res. 1, 2-11; Lin et al (2007) Cancer 109, 332-40) and metastatic progression (Brantley-Sieders et al (2005) FASEB J. 19, 1884-1886).

An antibody drug conjugate to EphA2 has been shown to significantly diminish tumour growth in rat and mouse xenograft models (Jackson et al (2008) Cancer Research 68, 9367-9374) and a similar approach has been tried in man although treatment had to be discontinued for treatment related adverse events (Annunziata et al (2013) Invest New drugs 31, 77-84).

Polypeptide ligands selected according to the method of the present invention may be employed in in vivo therapeutic and prophylactic applications, in vitro and in vivo diagnostic applications, in vitro assay and reagent applications, and the like. Ligands having selected levels of specificity are useful in applications which involve testing in non-human animals, where cross-reactivity is desirable, or in diagnostic applications, where cross-reactivity with homologues or paralogues needs to be carefully controlled. In some applications, such as vaccine applications, the ability to elicit an immune response to predetermined ranges of antigens can be exploited to tailor a vaccine to specific diseases and pathogens.

Substantially pure peptide ligands of at least 90 to 95% homogeneity are preferred for administration to a mammal, and 98 to 99% or more homogeneity is most preferred for pharmaceutical uses, especially when the mammal is a human. Once purified, partially or to homogeneity as desired, the selected polypeptides may be used diagnostically or therapeutically (including extracorporeally) or in developing and performing assay procedures, immunofluorescent stainings and the like (Lefkovite and Pernis, (1979 and 1981) Immunological Methods, Volumes I and II, Academic Press, NY).

According to a further aspect of the invention, there is provided a peptide ligand or a drug conjugate as defined herein, for use in preventing, suppressing or treating a disease or disorder characterised by overexpression of EphA2 in diseased tissue (such as a tumour).

According to a further aspect of the invention, there is provided a method of preventing, suppressing or treating a disease or disorder characterised by overexpression of EphA2 in diseased tissue (such as a tumour), which comprises administering to a patient in need thereof an effector group and drug conjugate of the peptide ligand as defined herein.

In one embodiment, the EphA2 is mammalian EphA2. In a further embodiment, the mammalian EphA2 is human EphA2.

In one embodiment, the disease or disorder characterised by overexpression of EphA2 in diseased tissue is selected from cancer.

Examples of cancers (and their benign counterparts) which may be treated (or inhibited) include, but are not limited to tumours of epithelial origin (adenomas and carcinomas of various types including adenocarcinomas, squamous carcinomas, transitional cell carcinomas and other carcinomas) such as carcinomas of the bladder and urinary tract, breast, gastrointestinal tract (including the esophagus, stomach (gastric), small intestine, colon, rectum and anus), liver (hepatocellular carcinoma), gall bladder and biliary system, exocrine pancreas, kidney, lung (for example adenocarcinomas, small cell lung carcinomas, non-small cell lung carcinomas, bronchioalveolar carcinomas and mesotheliomas), head and neck (for example cancers of the tongue, buccal cavity, larynx, pharynx, nasopharynx, tonsil, salivary glands, nasal cavity and paranasal sinuses), ovary, fallopian tubes, peritoneum, vagina, vulva, penis, cervix, myometrium, endometrium, thyroid (for example thyroid follicular carcinoma), adrenal, prostate, skin and adnexae (for example melanoma, basal cell carcinoma, squamous cell carcinoma, keratoacanthoma, dysplastic naevus); haematological malignancies (i.e. leukemias, lymphomas) and premalignant haematological disorders and disorders of borderline malignancy including haematological malignancies and related conditions of lymphoid lineage (for example acute lymphocytic leukemia [ALL], chronic lymphocytic leukemia [CLL], B-cell lymphomas such as diffuse large B-cell lymphoma [DLBCL], follicular lymphoma, Burkitt's lymphoma, mantle cell lymphoma, T-cell lymphomas and leukaemias, natural killer [NK] cell lymphomas, Hodgkin's lymphomas, hairy cell leukaemia, monoclonal gammopathy of uncertain significance, plasmacytoma, multiple myeloma, and post-transplant lymphoproliferative disorders), and haematological malignancies and related conditions of myeloid lineage (for example acute myelogenousleukemia [AML], chronic myelogenousleukemia [CML], chronic myelomonocyticleukemia [CMML], hypereosinophilic syndrome, myeloproliferative disorders such as polycythaemia vera, essential thrombocythaemia and primary myelofibrosis, myeloproliferative syndrome, myelodysplastic syndrome, and promyelocyticleukemia); tumours of mesenchymal origin, for example sarcomas of soft tissue, bone or cartilage such as osteosarcomas, fibrosarcomas, chondrosarcomas, rhabdomyosarcomas, leiomyosarcomas, liposarcomas, angiosarcomas, Kaposi's sarcoma, Ewing's sarcoma, synovial sarcomas, epithelioid sarcomas, gastrointestinal stromal tumours, benign and malignant histiocytomas, and dermatofibrosarcomaprotuberans; tumours of the central or peripheral nervous system (for example astrocytomas, gliomas and glioblastomas, meningiomas, ependymomas, pineal tumours and schwannomas); endocrine tumours (for example pituitary tumours, adrenal tumours, islet cell tumours, parathyroid tumours, carcinoid tumours and medullary carcinoma of the thyroid); ocular and adnexal tumours (for example retinoblastoma); germ cell and trophoblastic tumours (for example teratomas, seminomas, dysgerminomas, hydatidiform moles and choriocarcinomas); and paediatric and embryonal tumours (for example medulloblastoma, neuroblastoma, Wilms tumour, and primitive neuroectodermal tumours); or syndromes, congenital or otherwise, which leave the patient susceptible to malignancy (for example Xeroderma Pigmentosum).

In a further embodiment, the cancer is selected from: breast cancer, lung cancer, gastric cancer, pancreatic cancer, prostate cancer, liver cancer, glioblastoma and angiogenesis.

In a yet further embodiment, the cancer is selected from lung cancer, such as non-small cell lung carcinomas. Data is presented herein which demonstrates that a BDC of the invention (BDC-8) completely eradicated non-small cell lung carcinomas from day 32 and no tumour regrowth occurred following dosing suspension on day 28. This data clearly demonstrates the clinical utility of the BDCs of the present invention in cancers such as lung cancers, in particular non-small cell lung carcinomas.

References herein to the term “prevention” involves administration of the protective composition prior to the induction of the disease. “Suppression” refers to administration of the composition after an inductive event, but prior to the clinical appearance of the disease. “Treatment” involves administration of the protective composition after disease symptoms become manifest.

Animal model systems which can be used to screen the effectiveness of the peptide ligands in protecting against or treating the disease are available. The use of animal model systems is facilitated by the present invention, which allows the development of polypeptide ligands which can cross react with human and animal targets, to allow the use of animal models.

The invention is further described below with reference to the following examples.

EXAMPLE

Precurs Abbreviations Name Precursor Name or CAS Supplier 1Nal 1-Naphthylalanine Fmoc-3-(1-naphthyl)-L- 96402-49-2 Fluorochem alanine 2FuAla 2-Furylalanine Fmoc-L-2-furylalanine 159611-02-6 Combi Blocks 2Nal 2-Naphthylalanine Fmoc-3-(2-naphthyl)-L- 112883-43-9 Alfa Aesar alanine 3,3-DPA 3,3-Diphenylalanine fmoc-3,3-diphenylalanine 189937-46-0 Alfa Aesar 3,4-DCPhe 3,4- Fmoc-3,4-dichloro-L- 17766-59-5 PolyPeptide Dichlorophenylalanine phenylalanine 3Pal 3-(3-Pyridyl)- N-Fmoc-3-(3-pyridyl)- 175453-07-3 Fluorochem Alanine Lβnine 4,4-BPA 4,4′-Biphenylalanine Fmoc-L-4, 199110-64-0 Alfa Aesar 4′-Biphenylalanine 4BenzylPro 4-Benzyl- Fmoc-4-Benzyl- PolyPeptide pyrrolidine-2- pyrrolidine-2-carboxylic carboxylic acid acid 4BrPhe 4- Fmoc-4-Bromo-L- 198561-04-5 PolyPeptide Bromophenylalanine phenylalanine 4FlPro 4-Fluoro-pyrrolidine- Fmoc-4-fluoro-pyrrolidine- 203866-19-7 PolyPeptide 2-carboxylic acid 2-carboxylic acid 4MeoPhe 4- Fmoc-4- 77128-72-4 Iris Biotech Methoxyphenylalanine Methoxyphenylalanine 4Pal 3-(4-Pyridyl)- N-Fmoc-3-(4-pyridyl)-L- 169555-95-7 Fluorochem Alanine alanine 4PhenylPro 4-Phenyl- Fmoc-4-phenyl- 269078-71-9 Cambridge pyrrolidine-2- pyrrolidine-2-carboxylic Bioscience carboxylic acid acid Ac Acetyl AC3C 1-Aminocyclopropane- 1-(Fmoc-amino) 126705-22-4 Iris Biotech 1-carboxylic acid cyclopropanecarboxylic acid AC4C 1-Amino-1- 1-(Fmoc-amino)- 885951-77-9 Fluorochem cyclobutanecarboxylic acid cyclobutylcarboxylic acid AC5C 1-Amino-1- 1-(Fmoc-amino) 117322-30-2 Iris Biotech cyclopentanecarboxylic acid cyclopentanecarboxylic acid AF488 AlexaFluor488 AlexaFluor488-NHS Ester Fisher Scientific Aib 2-Aminoisobutyric Fmoc-α-aminoisobutyric 94744-50-0 Fluorochem acid acid Aza-Gly Azaglycine Aze Azetidine Fmoc-L-azetidine-2- 136552-06-2 Combi carboxylic acid Blocks β-Ala β-Alanine Fmoc-β-alanine 35737-10-1 Fluorochem C5g Cyclopentylglycine Fmoc-L- 220497-61-0 Fluorochem cyclopentylglycine Cba β-Cyclobutylalanine Fmoc-β-cyclobutyl-L- 478183-62-9 IRIS alanine Biotech GmbH Cpa β- Fmoc-β-cyclopropyl-L- 214750-76-2 Fluorochem Cyclopropylalanine alanine Cpg Cyclopropylglycine Fmoc-L- 1212257-18-5 Apollo cycloproprylglycine Scientific DOTA 1,4,7,10-tetraazacyclododecane-1,4,7,10- tetraacetic acid Fl 5(6)- Sigma carboxyfluorescein HArg HomoArginine Fmoc-L-HomoArg(Pbf)- 401915-53-5 Fluorochem OH HPhe HomoPhenylalanine Fmoc-L- 132684-59-4 Iris Biotech Homophenylalanine HyP Hydroxyproline Fmoc- 122996-47-8 Sigma Hydroxyproline(tBu)-OH NO2Phe 4- Fmoc-4-nitro-L- 95753-55-2 PolyPeptide Nitrophenylalanine phenylalanine Phg Phenylglycine Fmoc-L-phenylglycine 102410-65-1 Combi Blocks Pip Pipecolic acid Fmoc-L-Pipecolic acid 86069-86-5 Peptech Sar Sarcosine, such Fmoc-Sarcosine-OH 77128-70-2 Sigma that Sar_(x) represents x Sar residues tBuGly Tert-leucine Fmoc-L-tert-leucine 132684-60-7 Fluorochem Thi 2-Thienylalanine Fmoc-2-Thienylalanine 130309-35-2 Novabiochem ThiAz 3-(1,2,4-triazol-1- Fmoc-3-(1,2,4-triazol-1- 1217449-37-0 Sigma yl)-Alanine yl)-Ala-OH ΨAla Reduced amide on backbone

Materials and Methods

Peptide Synthesis

Peptide synthesis was based on Fmoc chemistry, using a Symphony and SymphonyX peptide synthesiser manufactured by Peptide Instruments and a Syro II synthesiser by MultiSynTech. Standard Fmoc-amino acids were employed (Sigma, Merck), with appropriate side chain protecting groups: where applicable standard coupling conditions were used in each case, followed by deprotection using standard methodology. Peptides were purified by HPLC and following isolation they were modified with 1,3,5-tris(bromomethyl)benzene (TBMB, Sigma). For this, linear peptide was diluted with H₂O up to ˜35 mL, ˜500 μL of 100 mM TBMB in acetonitrile was added, and the reaction was initiated with ˜5 mL of 1 M NH₄HCO₃ in H₂O. The reaction was allowed to proceed for ˜30-60 min at RT, and quenched with ˜500 ul of the 1M Cysteine hydrochloride (Sigma) once the reaction had completed (judged by MALDI). Following lyophilisation, the modified peptide was purified in a Gemini C18 column (Phenomenex) using water/acetonitrile with 0.1% trifluoroacetic acid as mobile phase. Pure fractions containing the correct cyclised material were pooled, lyophilised and kept at −20° C. for storage.

All amino acids, unless noted otherwise, were used in the L-configurations.

Preparation of Bicyclic Peptide Drug Conjugates BDC-1 to BDC-5

The synthesis of Bicyclic Peptide Drug Conjugates BDC-1 to BDC-5 listed in Tables 11 were performed using the protocol disclosed in WO 2016/067035.

Activated bicycle peptides with formula (C) and (D):

were synthesised by reacting the free amino group of the bicycle precursors with, respectively, SPP (N-succinimidyl 4-(2-pyridyldithio)pentanoate, Annova Chem) and SPDB (N-succinimidyl 3-(2-pyridyldithio)propionate, Annova Chem) in DMSO. Concentrations of bicycle precursors were 10 mM or higher, with a 1.3-fold excess of SPP or SPDB, and a 20-fold excess of diisopropylethylamine, at room temperature. The reaction was judged complete after 1 hour, as judged by LCMS. Purification was performed by reverse phase as described above. Appropriate fractions were lyophilised.

Activated bicycle peptides with formula (C) and (D) were disulphide exchanged with 1.15 equivalents of DM1 (as the free thiol), in semi aqueous conditions (50% dimethylacetamide and 50% 100 mM sodium acetate pH 5.0 supplemented with 2 mM EDTA) for 21 hours at room temperature under a nitrogen gas blanket. Concentrations of activated bicycle peptides with structure C and D in the reaction were at 10 mM or higher.

This was followed by standard reverse phase purification using a C18 column. Fractions at purity greater than 95% were isolated and lyophilised. The materials did not contain measurable quantities of free toxin.

BIOLOGICAL DATA 1. Fluorescence Polarisation Measurements

(a) Direct Binding Assay

Peptides with a fluorescent tag (either fluorescein, SIGMA or Alexa Fluor488™, Fisher Scientific) were diluted to 2.5 nM in PBS with 0.01% tween 20 or 50 mM HEPES with 100 mM NaCl and 0.01% tween pH 7.4 (both referred to as assay buffer). This was combined with a titration of protein in the same assay buffer as the peptide to give 1 nM peptide in a total volume of 25 μL in a black walled and bottomed low bind low volume 384 well plates, typically 5 μL assay buffer, 10 μL protein (Table 1) then 10 μL fluorescent peptide. One in two serial dilutions were used to give 12 different concentrations with top concentrations ranging from 500 nM for known high affinity binders to 10 μM for low affinity binders and selectivity assays. Measurements were conducted on a BMG PHERAstar FS equipped with an “FP 485 520 520” optic module which excites at 485 nm and detects parallel and perpendicular emission at 520 nm. The PHERAstar FS was set at 25° C. with 200 flashes per well and a positioning delay of 0.1 second, with each well measured at 5 to 10 minute intervals for 60 minutes. The gain used for analysis was determined for each tracer at the end of the 60 minutes where there was no protein in the well. Data was analysed using Systat Sigmaplot version 12.0. mP values were fit to a user defined quadratic equation to generate a Kd value: f=ymin+(ymax−ymin)/Lig*((x+Lig+Kd)/2-sqrt((((x+Lig+Kd)/2){circumflex over ( )}2)−(Lig*x))). “Lig” was a defined value of the concentration of tracer used.

(b) Competition Binding Assay

Peptides without a fluorescent tag were tested in competition with a peptide with a fluorescent tag and a known Kd (Table 2). Peptides were diluted to an appropriate concentration in assay buffer as described in the direct binding assay with a maximum of 5% DMSO, then serially diluted 1 in 2. Five μL of diluted peptide was added to the plate followed by 10 μL of human or mouse EphA2 (Table 1) at a fixed concentration which was dependent on the fluorescent peptide used (Table 2), then 10 μL fluorescent peptide added. Measurements were conducted as for the direct binding assay, however the gain was determined prior to the first measurement. Data analysis was in Systat Sigmaplot version 12.0 where the mP values were fit to a user defined cubic equation to generate a Ki value: f=ymin+(ymax−ymin)/Lig((Lig*((2*((Klig+Kcomp+Lig+Comp−Prot*c){circumflex over ( )}2−3*(Kcomp*(Lig−Prot*c)+Klig*(Comp−Prot*c)+Klig*Kcomp)){circumflex over ( )}0.5*COS(ARCCOS((−2*(Klig+Kcomp+Lig+Comp−Prot*c){circumflex over ( )}3+9*(Klig+Kcomp+Lig+Comp-Prot*c)*(Kcomp*(Lig−Prot*c)+Klig*(Comp−Prot*c)+Klig*Kcomp)−27*(−1*Klig*Kcomp*Prot*c))/(2*((((Klig+Kcomp+Lig+Comp−Prot*c){circumflex over ( )}2−3* (Kcomp*(Lig−Prot*c)+Klig*(Comp−Prot*c)+Klig*Kcomp)){circumflex over ( )}3){circumflex over ( )}0.5)))/3))−(Klig+Kcomp+Lig+Comp−Prot*c)))/((3*Klig)+((2*((Klig+Kcomp+Lig+Comp−Prot*c){circumflex over ( )}2−3*(Kcomp*(Lig−Prot*c)+Klig*(Comp−Prot*c)+Klig*Kcomp)){circumflex over ( )}0.5*COS(ARCCOS((−2*(Klig+Kcomp+Lig+Comp−Prot*c){circumflex over ( )}3+9*(Klig+Kcomp+Lig+Comp−Prot*c)*(Kcomp*(Lig−Prot*c)+Klig*(Comp−Prot*c)+Klig*Kcomp)−27*(−1*Klig*Kcomp*Prot*c))/(2*(MKlig+Kcomp+Lig+Comp−Prot*c){circumflex over ( )}2−3*(Kcomp*(Lig−Prot*c)+Klig*(Comp−Prot*c)+Klig*Kcomp)){circumflex over ( )}3){circumflex over ( )}0.5)))/3))−(Klig+Kcomp+Lig+Comp−Prot*c)))). “Lig”, “KLig” and “Prot” were all defined values relating to: fluorescent peptide concentration, the Kd of the fluorescent peptide and EphA2 concentration respectively.

TABLE 1 Ephrin receptors and source Receptor Catalogue (domain) Species Format/tag Supplier number EphA1 (Ecto) Human Fc fusion R&D systems 7146-A1 EphA2 (Ecto) Human C-terminal R&D systems 3035-A2 polyHis EphA2 (Ecto) Human C-terminal In-house N/A polyHis EphA2 (Ecto) Mouse Fc fusion R&D Systems 639-A2 EphA2 (Ecto) Mouse C-terminal Sino Biological 50586-M08H polyHis EphA2 (ligand Rat C-terminal In-house N/A binding) polyHis EphA2 (ligand Dog C-terminal In-house N/A binding) polyHis EphA3 (Ecto) Human Fc fusion R&D systems 6444-A3 EphA3 (Ecto) Human N-terminal In-house N/A polyHis EphA3 (Ecto) Rat C-terminal Sino Biological 80465-R08H polyHis EphA4 (Ecto) Human Fc fusion R&D systems 6827-A4 EphA4 (Ecto) Human C-terminal Sino Biological 11314-H08H polyHis EphA4 (Ecto) Rat C-terminal Sino Biological 80123-R08H polyHis EphA6 (Ecto) Human Fc fusion R&D systems 5606-A6 EphA7 (Ecto) Human Fc fusion R&D systems 6756-A7 EphB1 (Ecto) Rat Fc fusion R&D systems 1596-B1 EphB4 (Ecto) human C-terminal R&D systems 3038-B4 polyHis

TABLE 2 Final concentrations of fluorescent peptide and EphA2 as used with Competition Binding Assays Concentration Concentration Concentration Fluorescent of fluorescent of Human of Mouse peptide peptide (nM) EphA2 (nM) EphA2 (nM) Compound 1 1 300 Compound 12 10 75 Compound 66 1 30 Compound 18 0.8 (human) 1 2.4 50 (mouse)

The peptide ligands of the invention were tested in the above mentioned assays and the results are shown in Tables 3-12:

TABLE 3 Biological Assay Data for TBMB Peptide Ligands of the Invention (Direct Binding Assay) Bicycle K_(D), nm ± 95% Cl Compound  Mouse Number Sequence Human EphA2 EphA2  1 ACMNDWLCSLGVVTCA-Sar₆-K(FI) ((SEQ ID NO: 1-Sar₆-K(FI)) 107.58 ± 40.83 301 n = 1  2 (AF488-G-Sar₁₀-ACMNDWLCSLGWTC (AF488-G-Sar₁₀-SEQ ID NO: 2)  326 n = 1  3 ACMNDWLCELGWTCA-Sar₆-K(FI) ((SEQ ID NO: 3-Sar₆-K(FI)) 121.48 ± 50.27  4 ACTRQGIWCALGFEPCA-Sar₆-K(FI) ((SEQ ID NO: 4)-Sar₆-K(FI))  163.5 ± 22.54  5 ACMNDWLCTLGWSCA-Sar₆-K(FI) ((SEQ ID NO: 5)-Sar₆-K(FI))  142.5 ± 83.3  6 ACMNDWLCQLGWTCA-Sar₆-K(FI) ((SEQ ID NO: 6)-Sar₆-K(FI))   4.25 ± 4.8  7 ACMNDWLCTLGWTCA-Sar₆-K(FI) ((SEQ ID NO: 7)-Sar₆-K(FI))  74.35 ± 15.97  8 ACMNDWLCDLGWRCA-Sar₆-K(FI) ((SEQ ID NO: 8)-Sar₆-K(FI))  118.5 ± 22.54  9 ACMNDWLCELGWSCA-Sar₆-K(FI) ((SEQ ID NO: 9)-Sar₆-K(FI))  137.5 ± 49.98 10 ACRVSPEYCPFGPVWCAGAAA-Sar₆-K(FI) ((SEQ ID NO: ₁₀)-Sar6-K(FI)) 135.13 ± 59.02 11 FI-G-Sar₅-ACPWGPAWCPVHGKTCA (FI-G-Sar₅-(SEQ ID NO: 11))    263 ± 213.64 12 FI-G-Sar₅-ACPWGPAWCPVNRPGCA (FI-G-Sar₅-(SEQ ID NO: 12))  27.78 ± 8.35 13 Ac-ACPWGPAWCPVNRPGCAGAAA-K(FI) (Ac-(SEQ ID NO: 13)-K(FI))     29 ± 2.55 14 AF488-G-Sar₁₀-ACPWGPAWCPVNRPGCA (AF488-G-Sar₁₀-(SEQ ID NO: 12))   38 n = 1 15 FI-G-Sar₅-ACPWGPMWCPVNRPGCA (FI-G-Sar₅-(SEQ ID NO: 14))   12.6 ± 2.55 16 FI-G-Sar₅-ACPWGPNWCPVNRPGCA (FI-G-Sar₅-(SEQ ID NO: 15))   11.5 ± 1.76 17 FI-G-Sar₅-AGEMACPWGPFWCPVNRPGCA (FI-G-Sar₅-(SEQ ID NO: 16))   3.85 ± 0.1 18 FI-G-Sar₅-ADVTCPWGPFWCPVNRPGCA (FI-G-Sar₅-(SEQ ID NO: 17))   0.93 ± 0.23  4.02 ± 2 19 FI-G-Sar₅-ADVRTCPWGPFWCPVNRPGCA (FI-G-Sar₅-(SEQ ID NO: 18))   4.74 ± 0.51 20 FI-G-Sar₅-ANDVTCPWGPFWCPVNRPGCA (FI-G-Sar₅-(SEQ ID NO: 19))   2.35 ± 0.49 21 ACVPQGIWCALQFEPCA-Sar₆-K(FI) ((SEQ ID NO: 20)-Sar₆-K(FI))   59.5 ± 12.78 22 ACQKQGLWCALGFEPCA-Sar₆-K(FI) ((SEQ ID NO: 21)-Sar₆-K(FI))    289 ± 74.51 23 ACLVNDDCFYMGLCA-Sar₆-K(FI) ((SEQ ID NO: 22)-Sar₆-K(FI)) 109.38 ± 20.75

TABLE 5 Biological Assay Data for TBMB Peptide Ligands of the Invention (Competition Binding Assay) Bicycle Human EphA2 (K_(i), nM ± 95% Cl) Compound Fluroescent Peptide Number Sequence Compound 66 Compound 1 Compound 12 Compound 18 24 ACMNDWLCSLGWTCA  82.34 ± 12.8 (SEQ ID NO: 1) 25 Ac-CANDWLCSLGWTC  328 n = 1 (Ac-(SEQ ID NO: 23)) 26 Ac-CMNDWLCALGWTC   71.6 ± 3.33 (Ac-(SEQ ID NO: 24)) 27 Ac-CMNDWLCSAGWTC  356 n = 1 (Ac-(SEQ ID NO: 25)) 28 ACMNDWLCQLGWKCA  113 n = 1 (SEQ ID NO: 26) 29 ACMNDWLCELGWTCA  134.5 ± 32.34 (SEQ ID NO: 3) 30 ACMNDWLCQLGWTCA  56.05 ± 3.23 (SEQ ID NO: 6) 31 ACTQNDWLCSLGWTCA 151.65 ± 161.4 (SEQ ID NO: 27) 32 ACRNIPTMCPFGPVWCA 83.4 n = 1 (SEQ ID NO: 28) 33 ACRVSPEYCPFGPVWCA  78.53 ± 35.61 (SEQ ID NO: 29) 34 ACRVSPEYCPFGPVWCAGAAA   77.4 ± 8.95 (SEQ ID NO: 10) 35 ACRVSPEYCPFGPTWCA   43.2 ± 13.33 (SEQ ID NO: 30) 36 ACRVSPEYCPFGPSWCA   40.5 ± 5.88 (SEQ ID NO: 31) 37 ACRVSPEYCPFGPEWCA  61.25 ± 41.85 (SEQ ID NO: 32) 38 ACRVSPEYCPFGPYWCA  26.53 ± 16.92 (SEQ ID NO: 33) 39 ACRVSPEYCPFGPLWCA  32.11 ± 10.28 (SEQ ID NO: 34) 40 ACRVSPEYCPFGPDWCA   55.4 ± 9.41 (SEQ ID NO: 35) 41 ACPWGPAWCPVHGKTCA  263 n = 1 (SEQ ID NO: 11) 42 ACPWGPAWCPVRDTNCA  316 n = 1 (SEQ ID NO: 36) 43 ACPWGPAWCPVNGARCA  430 n = 1 (SEQ ID NO: 37) 44 ACPWGPAWCPVNRPGCA 191.22 ± 29.47  164 n = 1 128.45 ± 28.21 (SEQ ID NO: 12) 45 ACPWGPAWCPVNRPGCAGAAA 117.13 ± 17.96  99.15 ± 48.71 (SEQ ID NO: 13) 46 ACPWGPMWCPVNRPGCA  95.75 ± 29.89 (SEQ ID NO: 14) 47 ACPWGPNWCPVNRPGCA  78.35 ± 12.64 (SEQ ID NO: 15) 48 ACPWGPAWCPVRNPCA    284 ± 47.04 (SEQ ID NO: 38) 49 ACPWGPAWCPVSRVCA    428 ± 99.96 (SEQ ID NO: 39) 50 ACPWGPAWCPVRSCA    314 ± 248.92 (SEQ ID NO: 40) 51 ACPWGPAWCPVKPTCA  318.5 ± 255.78 (SEQ ID NO: 41) 52 ACPWGPAWCPVNRNGCA    168 ± 72.52 (SEQ ID NO: 42) 53 AGEMACPWGPFWCPVNRPGCA      6 ± 5.54 12.33 ± 2 (SEQ ID NO: 16) 54 AVHIPCPWGPSWCPVNRPGCA   5.17 ± 2.76  5.13 ± 1.52 (SEQ ID NO: 43) 55 AEGLPCPWGPFWCPVNRPGCA   6.15 ± 3.43  11.3 ± 2.04 (SEQ ID NO: 44) 56 ADHACPWGPFWCPVNRPGCA   5.87 ± 5.09 14.43 ± 6.28 (SEQ ID NO: 45) 57 ADVHCPWGPFWCPVNRPGCA  1.2 n = 1  0.48 ± 0.15 (SEQ ID NO: 46) 58 ADVTCPWGPFWCPVNRPGCA   2.65 ± 1.08  1.35 ± 0.23 (SEQ ID NO: 17) 59 AHDVPCPWGPFWCPVNRPGCA  0.54 ± 0.14 (SEQ ID NO: 47) 60 ADVRTCPWGPFWCPVNRPGCA  2.5 n = 1 12.63 ± 1.29 (SEQ ID NO: 18) 61 ANDVTCPWGPFWCPVNRPGCA  7.3 n = 1  2.93 ± 0.07 (SEQ ID NO: 19) 62 ARDDPCPWGPFWCPVNRPGCA  27.96 ± 16.74 16.13 ± 0.8 (SEQ ID NO: 48) 63 ACVPQGIWCALQFEPCA  82.45 ± 27.07  144 n = 1   92.2 ± 21.17 (SEQ ID NO: 20) 64 ACTTGSIWCALQFEPCA 63.4 n = 1  410 n = 1 (SEQ ID NO: 49) 65 ACVPQGIWCALRYEPCA  293 n = 1  229 n = 1 (SEQ ID NO: 50)

TABLE 5 Biological Assay Data for TBMB Peptide Ligands of the Invention (Direct Binding Assay) Bicycle K_(D), nM ± 95% Cl Compound Human Number Sequence EphA2 Mouse EphA2 66 Fl-G-Sar₅-ACPWGPFWCPVNRPGCA (Fl-G-Sar₅-(SEQ ID NO: 51)) 8.45 ± 0.4 22 n = 1 67 AlexaFluor488-G-Sar₅-ACPWGPFWCPVNRPGC (AlexaFluor488-G-Sar₅- 15.03 ± 1.72 51.8 ± 6.27 (SEQ ID NO: 52)) 68 AlexaFluor488-(β-Ala)-Sar₁₀-ACPWGPFWCPVNRPGC (AlexaFluor488- 15.37 ± 2.87 23.4 n = 1 ((β-Ala)-Sar₁₀-(SEQ ID NO: 52))

TABLE 6 Biological Assay Data for TBMB Peptide Ligands of the Invention (Competition Binding Assay) Bicycle Ki, nM ± 95% Cl Com- Human EphA2 pound Sequence Fluorescent Peptide Number Compound 18 Compound 66 69 ACPWGPFWCPVNRPGCA (SEQ ID NO: 51) 106.75 ± 44.25  70.08 ± 8.01 70 Sar₂-ACPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 52))  51.81 ± 21.75  20.45 ± 12.84 71 Ac-Sar₂-ACPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 52))  11.87 ± 7.51 72 (β-Ala)-Sar₁₀-ACPWGPFWCPVNRPGC ((β-Ala)-Sar₁₀-(SEQ ID NO: 52))   29.1 ± 5.08  20.98 ± 2.18 73 Sar₂-AC(HyP)WGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 53))   47.6 ± 18.42  247.5 ± 18.62 74 Sar₂-AC(Aib)WGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 54))  138.9 ± 88.79 75 Sar₂-AC(4FlPro)WGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 55)) 399.67 ± 90.63 76 Sar₂-ACP(1Nal)GPFWCPVNRPGC (Sar₂-(SEQ ID NO: 56))    3.5 ± 1.96   16.7 ± 9.68 77 Sar₂-ACP(2Nal)GPFWCPVNRPGC (Sar₂-(SEQ ID NO: 57)) 458.33 ± 222.44 78 Sar₂-ACPWG(Aze)FWCPVNRPGC (Sar₂-(SEQ ID NO: 58))  403.5 ± 12.74 79 Sar₂-ACPWG(HyP)FWCPVNRPGC (Sar₂-(SEQ ID NO: 59))    131 ± 22.97 80 Sar₂-ACPWG(Aib)FWCPVNRPGC (Sar₂-(SEQ ID NO: 60))  120.5 ± 81.34 186.73 ± 94.37 81 Sar₂-ACPWG(4FlPro)FWCPVNRPGC (Sar₂-(SEQ ID NO: 61))    294 ± 99.6 82 Sar₂-ACPWG(Pip)FWCPVNRPGC (Sar₂-(SEQ ID NO: 62)) 497.33 ± 223.62 83 Sar₂-ACPWGPAWCPVNRPGC (Sar₂-(SEQ ID NO: 63)) 199 n = 1  287.5 ± 197.95 84 Sar₂-ACPWGP(4Pal)WCPVNRPGC (Sar₂-(SEQ ID NO: 64))   33.5 ± 0.98  81.47 ± 68.95 85 Sar₂-ACPWGP(4BrPhe)WCPVNRPGC (Sar₂-(SEQ ID NO: 65))  174.5 ± 20.58 86 Sar₂-ACPWGP(4MeOPhe)WCPVNRPGC (Sar₂-(SEQ ID NO: 66))  274.5 ± 36.26 87 Sar₂-ACPWGP(HPhe)WCPVNRPGC (Sar₂-(SEQ ID NO: 67)) 162 n = 1  281.2 ± 154.82 88 Sar₂-ACPWGP(4,4-BPA)WCPVNRPGC (Sar₂-(SEQ ID NO: 68)) 182.67 ± 99.5 89 Sar₂-ACPWGP(NO2Phe5)WCPVNRPGC (Sar₂-(SEQ ID NO: 69))  289.5 ± 93.1 90 Sar₂-ACPWGP(3,4-DCPhe)WCPVNRPGC (Sar₂-(SEQ ID NO: 70))    361 ± 25.48 91 Sar₂-ACPWGPYWCPVNRPGC (Sar₂-(SEQ ID NO: 71)) 137.63 ± 104.2 92 Sar₂-ACPWGP(3Pal)WCPVNRPGC (Sar₂-(SEQ ID NO: 72))    165 ± 27.44 93 Sar₂-ACPWGP(Phg)WCPVNRPGC (Sar₂-(SEQ ID NO: 73))  411.5 ± 128.38 94 Sar₂-ACPWGP(1Nal)WCPVNRPGC (Sar₂-(SEQ ID NO: 74))  196.5 ± 6.86 95 Sar₂-ACPWGP(2Nal)WCPVNRPGC (Sar₂-(SEQ ID NO: 75))  362.5 ± 110.74 96 Sar₂-ACPWGPF(1Nal)CPVNRPGC (Sar₂-(SEQ ID NO: 76))   31.3 ± 24.11  68.13 ± 35.66 97 Sar₂-ACPWGPFWC(Aze)VNRPGC (Sar₂-(SEQ ID NO: 77))    286 ± 109.76 98 Sar₂-ACPWGPFWC(HyP)VNRPGC (Sar₂-(SEQ ID NO: 78)) 163.33 ± 38.41 99 Sar₂-ACPWGPFWC(4FlPro)VNRPGC (Sar₂-(SEQ ID NO: 79))  269.5 ± 6.86 100 Sar₂-ACPWGPFWCP(tBuGly)NRPGC (Sar₂-(SEQ ID NO: 80))   58.3 ± 50.37 112.45 ± 73.38 101 Sar₂-ACPWGPFWCPVARPGC (Sar₂-(SEQ ID NO: 81)) 293 n = 1    265 ± 235.04 102 Sar₂-ACPWGPFWCPV(D-Ala)RPGC (Sar₂-(SEQ ID NO: 82))    317 ± 168.56 311.67 ± 195.55 103 Sar₂-ACPWGPFWCPVN(HArg)PGC (Sar₂-(SEQ ID NO: 83))    126 ± 9.8 169.43 ± 94.28 104 Sar₂-ACPWGPFWCPVNRAGC (Sar₂-(SEQ ID NO: 84)) 124 n = 1 193.67 ± 112.76 105 Sar₂-ACPWGPFWCPVNR(D-Ala)GC (Sar₂-(SEQ ID NO: 85)) 470.67 ± 221.53 106 Sar₂-ACPWGPFWCPVNR(Aze)GC (Sar₂-(SEQ ID NO: 86))    155 ± 47.04 107 Sar₂-ACPWGPFWCPVNR(HyP)GC (Sar₂-(SEQ ID NO: 87)) 48.7 n = 1  85.83 ± 57.98 108 Sar₂-ACPWGPFWCPVNR(Pip)GC (Sar₂-(SEQ ID NO: 88))  374.5 ± 12.74 109 Sar₂-ACPWGPFWCPVNR(4FlPro)GC (Sar₂-(SEQ ID NO: 89))  184.5 ± 20.58 110 Sar₂-ACPWGPFWCPVNR(Aib)GC (Sar₂-(SEQ ID NO: 90))     75 ± 13.72 139.53 ± 103.98 111 Sar₂-ACPWGPFWCPVNRPAC (Sar₂-(SEQ ID NO: 91)) 108 n = 1  237.5 ± 164.92 112 Sar₂-ACPWGPFWCPVNRP(D-Ala)C (Sar₂-(SEQ ID NO: 92))     89 ± 15.68 113 Sar₂-AC(Aib)(1Nal2)GPFWCPVNRPGC (Sar₂-(SEQ ID NO: 93)) 10 n = 1 6.6 n = 1 114 Sar₂-AC(Aib)WGPF(1Nal)CPVNRPGC (Sar₂-(SEQ ID NO: 94)) 21 n = 1 43 n = 1 115 Sar₂-ACP(1Nal)GPFWCPV(D-Ala)RPGC (Sar₂-(SEQ ID NO: 95))   12.5 ± 0.98   1.64 ± 2.48 116 Sar₂-ACP(1Nal)GPFWCPVNRP(D-Ala)C (Sar₂-(SEQ ID NO: 96))   2.95 ± 1.67 3.2 n = 1 117 Sar₂-ACPWGPF(1Nal)CPV(D-Ala)RPGC (Sar₂-(SEQ ID NO: 97)) 53 n = 1 75 n = 1 118 Sar₂-ACPWGPF(1Nal)CPVNRP(D-Ala)C (Sar₂-(SEQ ID NO: 98)) 37 n = 1     18 ± 13.72 119 Sar₂-ACP(1Nal)G(Aib)FWCPVNRPGC (Sar₂-(SEQ ID NO: 99)) 21 n = 1 8.4 n = 1 120 Sar₂-ACP(1Nal)GPF(1Nal)CPVNRPGC (Sar₂-(SEQ ID NO: 100))    1.4 ± 0.39 0.98 n = 1 121 Sar₂-ACP(1Nal)GPFWCP(tBuGly)NRPGC (Sar₂-(SEQ ID NO: 101))   3.65 ± 0.29 2 n = 1 122 Sar₂-ACP(1Nal)GPFWCPVN(HArg)PGC (Sar₂-(SEQ ID NO: 102))   9.55 ± 0.69 8 n = 1 123 Sar₂-ACPWG(Aib)F(1Nal)CPVNRPGC (Sar₂-(SEQ ID NO: 103)) 63 n = 1 46 n = 1 124 Sar₂-AC(Aib)(1Nal)GPFWCPV(D-Ala)RPGC (Sar₂-(SEQ ID NO: 104)) 26 n = 1 2.5 n = 1 125 Sar₂-AC(Aib)(1Nal)GPFWCPVNRP(D-Ala)C (Sar₂-(SEQ ID NO: 105))    6.4 ± 0.78   0.61 ± 0.96 126 Sar₂-ACP(1Nal)G(Aib)FWCP(tBuGly)NRPGC (Sar₂-(SEQ ID NO: 106)) 15 n = 1 19 n = 1 127 Sar₂-ACP(1Nal)G(Aib)FWCPV(D-Ala)RPGC (Sar₂-(SEQ ID NO: 107)) 40 n = 1 33 n = 1 128 Sar₂-ACP(1Nal)G(Aib)FWCPVNRP(D-Ala)C (Sar₂-(SEQ ID NO: 108)) 15 n = 1 16 n = 1 129 Sar₂-ACP(1Nal)GPFWCP(tBuGly)(D-Ala)RPGC (Sar₂-(SEQ ID NO: 109)) 23 n = 1 15 n = 1 130 Sar₂-ACP(1Nal)GPFWCP(tBuGly)N(HArg)PGC (Sar₂-(SEQ ID NO: 110))   0.29 ± 0.34 131 Sar₂-ACP(1Nal)GPFWCP(tBuGly)NR(Aib)GC (Sar₂-(SEQ ID NO: 111)) 11 n = 1 6.8 n = 1 132 Sar₂-ACP(1Nal)GPFWCP(tBuGly)NRP(D-Ala)C (Sar₂-(SEQ ID NO: 112))    7.7 ± 1.96 8.7 n = 1 133 Sar₂-ACP(1Nal)GPFWCPV(D-Ala)(HArg)PGC (Sar₂-(SEQ ID NO: 113)) 14 n = 1 3.7 n = 1 134 Sar₂-ACP(1Nal)GPFWCPVN(HArg)P(D-Ala)C (Sar₂-(SEQ ID NO: 114)) 1.2 n = 1   6.15 ± 0.29 135 Sar₂-AC(Aib)(1Nal)G(Aib)FWCPVNR(Aib)GC (Sar₂-(SEQ ID NO: 115)) 43 n = 1 30 n = 1 136 Sar₂-ACP(1Nal)G(Aib)FWCP(tBuGly)N(HArg)PGC (Sar₂-(SEQ ID NO: 116)) 23 n = 1 15 n = 1 137 Sar₂-ACP(1Nal)G(Aib)FWCP(tBuGly)NR(Aib)GC (Sar₂-(SEQ ID NO: 117)) 20 n = 1 18 n = 1 138 Sar₂-ACP(1Nal)GPFWCP(tBuGly)N(HArg)(Aib)GC (Sar₂-(SEQ ID NO: 118)) 5.1 n = 1

TABLE 7 Biological Assay Data for TBMB Peptide Ligands of the Invention (Direct Binding Assay) Bicycle Com- pound K_(D), nM ± 95% Cl Number Sequence Human EphA2 Mouse EphA2 139 AF488-(β-Ala)-Sar₁₀-H(D-Asp)VPCPWGPFWCPVNRPGCA (AF488-(β-Ala)-Sar₁₀-(SEQ 0.31 ± 0.18  0.8 ± 0.54 ID NO: 119)) 140 AF488-(β-Ala)-Sar₁₀-H(D-Asp)VP-C(Aib)(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg) 2.05 ± 0.62 4.55 ± 1.04 P(D-Asp)C (AF488-(β-Ala)-Sar₁₀-(SEQ ID NO: 120)) 141 AF488-(β-Ala)-Sar₁₀-H(D-Asp)VP-C(Aib)(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib) 2.65 ± 0.64  6.5 ± 0.63 (D-Asp)C (AF488-(β-Ala)-Sar₁₀-(SEQ ID NO: 121)) 142 Fl-(β-Ala)-Sar₁₀-H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)P(D-Asp) 1.7 n = 1 C (Fl-(β-Ala)-Sar₁₀-(SEQ ID NO: 122)) 143 AF488-(β-Ala)-Sar₁₀-H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)P  1.4 ± 1.46 4.69 ± 4.15 (D-Asp)C(AF488-(β-Ala)-Sar₁₀-(SEQ ID NO: 122)) 144 AF488-(β-Ala)-Sar₁₀-H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)GC 1.04 n = 1 2.56 n = 1 (AF488-(β-Ala)-Sar₁₀-(SEQ ID NO: 123)) 145 AF488-(β-Ala)-Sar₁₀-H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib) 2.17 ± 2.08  3.8 ± 0.55 (D-Asp)C(AF488-(β-Ala)-Sar₁₀-(SEQ ID NO: 124)) 146 AF488-(β-Ala)-Sar₁₀-H(D-Asp)(C5g)P-C(Aib)(1Nal)G(Aib)F(1Nal)CP(tBuGly) 2.19 n = 1 NR(Aib)(D-Asp)C (AF488-(β-Ala)-Sar₁₀-(SEQ ID NO: 125)) 147 AF488-(β-Ala)-Sar₁₀-H(D-Asp)(C5g)P-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N 1.07 ± 0.9 3.44 ± 1.31 (HArg)P(D-Asp)C (AF488-(β-Ala)-Sar₁₀-(SEQ ID NO: 126))

TABLE 8 Biological Assay Data for TBMB Peptide Ligands of the Invention (Competition Binding Assay) Ki, nM ± 95% Cl Bicycle Fluorescent peptide Compound Human EphA2 Mouse EphA2 Number Sequence Compound 18 Compound 18 148 Ac-Sar₂-ADVH-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 127))    1.2 ± 0.2 149 ADVH-CP(3,3-DPA)GPFWCPVNRPGCA (SEQ ID NO: 128)   52.8 ± 11.6 150 ADVH-CP(1Nal)GPFWCPVNRPGCA (SEQ ID NO: 129)   0.12 ± 0.07 151 ADVH-CPWAPFWCPVNRPGCA (SEQ ID NO: 130)  393.5 ± 206.78 152 ADVH-CPWGAFWCPVNRPGCA (SEQ ID NO: 131)    1.8 ± 0.74 153 ADVH-CPWG(Aib)FWCPVNRPGCA (SEQ ID NO: 132)   0.51 ± 0.29 154 ADVH-CPWGPFWCAPVNRPGCA (SEQ ID NO: 133) 101.03 ± 33.68 155 ADVH-CPWGPFWCPV(D-Ala)RPGCA (SEQ ID NO: 134)      2 ± 0.74 156 ADVH-CPWGPFWCPVN(D-Ala)PGCA (SEQ ID NO: 135)  14.93 ± 2.3 157 Ac-Sar₂-ADVT-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 136))   0.91 ± 0.19 158 Ac-Sar₂-A(D-Asp)VT-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 137))   2.05 ± 0.42 2.2 ± 0.4 159 Ac-Sar₂-A(D-Asp)(D-Asp)T-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 138))   2.85 ± 0.49 160 Ac-Sar₂-A(D-Asp)(Cba)T-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 139))    2.6 ± 0.11 161 Ac-Sar₂-A(D-Asp)(Cpa)T-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 140))   4.44 ± 1.08 162 Ac-Sar₂-A(D-Asp)(Cpg)T-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 141))   2.55 ± 0.55 163 Ac-Sar₂-A(D-Asp)(C5g)VT-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 142))   1.33 ± 0.27 1.74 ± 1.23 164 Ac-Sar₂-AD(tBuGly)T-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 143))   2.25 ± 0.69 165 Ac-Sar₂-A(D-Asp)VT-C(AC3C)WGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 144))    185 ± 147 166 Ac-Sar₂-A(D-Asp)VT-C(AC4C)WGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 145))   76.7 ± 73.11 167 Ac-Sar₂-A(D-Asp)VT-C(AC5C)WGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 146)) 138 n = 1 168 Ac-Sar₂-A(D-Asp)VT-C(4BenzyPro)WGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 147))   5.03 ± 2.24 169 Ac-Sar₂-A(D-Asp)VT-C(4PhenyPro)WGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 148))   14.4 ± 7.64 170 Ac-Sar₂-A(D-Asp)VT-CP(1Nal)GPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 149))    0.6 ± 0.19 171 Ac-Sar₂-A(D-Asp)VT-CPWGP(HArg)WCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 150))   4.88 ± 2.19 172 Ac-Sar₂-A(D-Asp)VT-CPWGPNWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 151))   3.96 ± 0.72 173 Ac-Sar₂-A(D-Asp)VT-CPWGPAWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 152))   6.69 ± 3.49 174 Ac-Sar₂-A(D-Asp)VT-CPWGPFWCPLNRPGC (Ac-Sar₂-(SEQ ID NO: 153))    9.1 ± 1.73 175 Ac-Sar₂-A(D-Asp)VT-CPWGPFWCPVNRP(D-Asp)C (Ac-Sar₂-(SEQ ID NO: 154))   1.78 ± 0.54 176 Ac-Sar₂-A(D-Asp)VT-CPWGPFWCPVN(HArg)P(D-Asp)C (Ac-Sar₂-(SEQ ID NO: 155))   4.89 ± 0.97 177 Ac-Sar₂-A(D-Asp)VT-CPWGPFWCPVNR(Aib)(D-Asp)C (Ac-Sar₂-(SEQ ID NO: 156))   4.43 ± 2.37 178 Ac-Sar₂-A(D-Asp)VT-CP(1Nal)G(Aib)FWCPVNR(Aib)GC (Ac-Sar₂-(SEQ ID NO: 157))    2.4 ± 0.23 179 Ac-Sar₂-A(D-Asp)VT-CPWG(Aib)F(1Nal)CPVNR(Aib)GC (Ac-Sar₂-(SEQ ID NO: 158))   2.94 ± 0.09 180 Ac-Sar₂-A(D-Asp)VT-CPWG(Aib)FWCP(tBuGly)NR(Aib)GC (Ac-Sar₂-(SEQ ID NO:   3.83 ± 0.43 159)) 181 Ac-Sar₂-A(D-Asp)VT-CP(1Nal)G(Aib)F(1Nal)CPVNR(Aib)GC (Ac-Sar₂-(SEQ ID NO:   1.37 ± 0.41 160)) 182 Ac-Sar₂-A(D-Asp)VT-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)PGC (Ac-Sar₂-(SEQ   1.16 ± 0.39 ID NO: 161)) 183 Ac-Sar₂-A(D-Asp)VT-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)GC (Ac-Sar₂-(SEQ ID   1.02 ± 0.41 NO: 162)) 184 Ac-Sar₂-A(D-Asp)VT-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C (Ac-Sar₂-   1.19 ± 0.29 (SEQ ID NO: 163)) 185 (D-Asp)VT-CPWGPFWCPVNRPGC (SEQ ID NO: 164)   2.17 ± 0.73 186 (D-Asp)VT-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)GC (SEQ ID NO: 165)   1.34 ± 0.18 187 AHDVP-CP(1Nal)GPFWCPVNRPGCA (SEQ ID NO: 166)   0.32 ± 0.03 188 AHDVP-CP(1Nal)GPFWCPVNRPGC (SEQ ID NO: 167)   1.45 ± 0.1 189 AHDVP-CPWGPF(1Nal)CPVNRPGC (SEQ ID NO: 168)    1.3 ± 0.2 190 AHDVP-CP(1Nal)GPFWCP(tBuGly)NRPGC (SEQ ID NO: 169)    0.7 ± 0.4 191 AHDVP-CP(1Nal)G(Aib)FWCP(tBuGly)N(HArg)PGC (SEQ ID NO: 170)    3.1 ± 0.68 192 AHDVP-CP(1Nal)G(Aib)FWCP(tBuGly)NR(Aib)GC (SEQ ID NO: 171)   1.75 ± 0.1 193 Ac-Sar₂-AHDVP-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 172)   0.59 ± 0.2 194 Ac-Sar₂-(D-Ala)HDVP-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 173)    1.2 ± 0.39 195 Ac-Sar₂-AADVP-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 174)   1.01 ± 0.19 196 Ac-Sar₂-A(D-His)DVP-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 175)   0.95 ± 0.24 197 Sar₂-A(D-His)DVP-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 175)    1.2 198 Ac-Sar₂-A(D-His)DVCPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 176)     20 ± 1.96 199 Sar₂-A(D-Ala)DVP-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 177)   3.35 ± 1.47 200 Ac-Sar₂-A(D-Asp)DVP-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 178)    4.1 ± 0.2 201 Sar₂-A(ThODVP-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 179)    0.6 ± 0.04 202 Sar₂-A(ThiAz)DVP-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 180)    0.7 ± 0.08 203 Sar₂-A(2FuAla)DVP-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 181)   0.49 ± 0.24 204 Ac-Sar₂-A(D-His)D(tBuGly)P-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 182)   2.15 ± 0.1 205 Sar₂-AHAVP-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 183)    1.8 ± 0.2 206 Sar₂-AH(D-Ala)VP-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 184)    8.3 ± 0.78 207 Sar₂-AHEVP-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 185)    1.3 ± 0.39 208 Sar₂-AH(D-Glu)VP-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 186)      2 ± 0.39 209 Sar₂-AH(D-Asp)VP-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 187)   1.25 ± 0.29 210 Ac-Sar₂-AH(D-Asp)VP-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 187)    1.1 ± 0.2 211 Ac-Sar₂-AH(D-Asp)(tBuGly)P-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 188)    3.1 ± 0.2 212 Ac-Sar₂-AH(D-Asp)V(Sar)-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 189)   4.95 ± 1.86 213 Ac-Sar₂-AH(D-Asp)V(Aib)-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 190)    1.9 ± 0.2 214 Sar₂-AHDAP-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 191)   22.5 ± 2.94 215 Sar₂-AHD(D-Ala)P-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 192)     26 ± 7.84 216 Sar₂-AHD(Aib)P-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 193)   2.77 ± 0.24 217 Sar₂-AHD(tBuGly)P-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 194) 0.49 n = 1 218 Sar₂-AHDVA-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 195)   1.27 ± 0.07 219 Sar₂-AHDV(D-Ala)-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 196)     15 ± 3.92 220 Sar₂-AHDV(Aib)-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 197)   0.83 ± 0.15 221 Sar₂-AHDV(Aze)-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 198)    3.1 ± 0.39 222 Sar₂-AHDV(Pip)-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 199)    3.4 ± 0.2 223 (β-Ala)-Sar₁₀-HDVP-CPWGPFWCPVNRPGC ((β-Ala)-Sar₁₀-(SEQ ID NO: 200))   1.29 ± 0.42 224 Ac-Sar₂-(D-His)DVP-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 201))   1.09 ± 0.13 225 Ac-Sar₂-H(D-Asp)VP-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 202))      1 ± 0.18 2.08 ± 1.27 226 (β-Ala)-Sar₁₀-H(D-Asp)VP-CPWGPFWCPVNRPGC ((β-Ala)-Sar₁₀-(SEQ ID NO: 202))   0.84 ± 0.24 227 Ac-Sar₂-AH(D-Asp)VP-CP(1Nal)GPFWCP(tBuGly)N(HArg)PGC (Ac-Sar₂-(SEQ ID   0.75 ± 0.36 NO: 203)) 228 Ac-Sar₂-A(D-His)DVP-CPWGP(4)A1a)WCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 204))  210.5 ± 48.02 229 Ac-Sar₂-A(D-His)DVP-CPWGPFWCP(HArg)NRPGC (Ac-Sar₂-(SEQ ID NO: 205))    5.1 ± 1.18 230 Ac-Sar₂-A(D-His)DVP-CP(1Nal)GPFWCP(tBuGly)N(HArg)PGC (Ac-Sar₂-(SEQ ID NO:    1.8 ± 0.78 206)) 231 Ac-Sar₂-A(D-His)DVP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)PGC (Ac-Sar₂-(SEQ   1.93 ± 0.23 ID NO: 207)) 232 Ac-Sar₂-A(D-His)DVP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)GC (Ac-Sar₂-(SEQ    0.9 ± 0.68 ID NO: 208)) 233 Ac-Sar₂-A(D-His)DVP-CPWG(Aib)FWCP(tBuGly)NR(Aib)GC (Ac-Sar₂-(SEQ ID NO:    4.8 ± 0.84 209)) 234 (β-Ala)-Sar₁₀-H(D-Asp)VP-C(Aib)(1Nal)G(Aib)F(1Nal)CP(tBuGly)NRPGC ((β-Ala)-   3.94 ± 1.72 Sar₁₀-(SEQ ID NO: 210)) 235 (β-Ala)-Sar₁₀-H(D-Asp)VP-C(Aib)(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)PGC ((β-   2.58 ± 0.96 Ala)-Sar₁₀-(SEQ ID NO: 211)) 236 (β-Ala)-Sar₁₀-H(D-Asp)VP-C(Aib)(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C      3 ± 0.71 ((β-Ala)-Sar₁₀-(SEQ ID NO: 120)) 237 Ac-(β-Ala)-Sar₁₀-H(D-Asp)VP-C(Aib)(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)P(D- 2.4 n = 1 Asp)C (Ac-(β-Ala)-Sar₁₀-(SEQ ID NO: 120)) 238 (β-Ala)-Sar₁₀-H(D-Asp)VP-C(Aib)(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)(Aib)(D-   2.83 ± 0.19 Asp)C ((β-Ala)-Sar₁₀-(SEQ ID NO: 212)) 239 (β-Ala)-Sar₁₀-H(D-Asp)VP-C(Aib)(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)GC ((β-Ala)-   4.91 ± 2.45 Sar₁₀-(SEQ ID NO: 213)) 240 (β-Ala)-Sar₁₀-H(D-Asp)VP-C(Aib)(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)(D-Asp)C ((β- 2.41 n = 1 Ala)-Sar₁₀-(SEQ ID NO: 121)) 241 Ac-(β-Ala)-Sar₁₀-H(D-Asp)VP-C(Aib)(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)(D-Asp)C   2.98 ± 0.96 (Ac-(β-Ala)-Sar₁₀-(SEQ ID NO: 121)) 242 (β-Ala)-Sar₁₀-H(D-Asp)VP-C(Aib)(1Nal)G(Aib)FWCP(tBuGly)N(HArg)P(D-Asp)C ((β-   7.77 ± 3.02 Ala)-Sar₁₀-(SEQ ID NO: 214)) 243 (β-Ala)-Sar₁₀-H(D-Asp)VP-C(Aib)(1Nal)GP(HArg)(1Nal)CP(tBuGly)NR(Aib)GC ((β-   2.83 ± 0.72 Ala)-Sar₁₀-(SEQ ID NO: 215)) 244 (β-Ala)-Sar₁₀-H(D-Asp)VP-C(Aib)(1Nal)GP(HArg)(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C    2.8 ± 0.26 ((β-Ala)-Sar₁₀-(SEQ ID NO: 216)) 245 (β-Ala)-Sar₁₀-H(D-Asp)VP-C(Aib)WGP(HArg)(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C ((β-  11.91 ± 4.3 Ala)-Sar₁₀-(SEQ ID NO: 217)) 246 (β-Ala)-Sar₁₀-H(D-Asp)VP-C(Aib)WGP(HArg)WCP(tBuGly)N(HArg)P(D-Asp)C ((β-   31.4 ± 24.3 Ala)-Sar₁₀-(SEQ ID NO: 218)) 247 (β-Ala)-Sar₁₀-H(D-Asp)VP-C(Aib)WGP(HArg)WCP(tBuGly)N(HArg)P(D-Asp)C ((β-   29.7 ± 11.76 Ala)-Sar₁₀-(SEQ ID NO: 218)) 248 (β-Ala)-Sar₁₀-H(D-Asp)VP-C(D-Ala)(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C   28.4 ± 0.78 ((β-Ala)-Sar₁₀-(SEQ ID NO: 219)) 249 (β-Ala)-Sar₁₀-H(D-Asp)VP-CP(1Nal)G(Aib)(HArg)(1Nal)CP(tBuGly)NR(Aib)GC ((β-   1.38 ± 0.46 Ala)-Sar₁₀-(SEQ ID NO: 220)) 250 (β-Ala)-Sar₁₀-H(D-Asp)VP-CP(1Nal)G(Aib)(HArg)(1Nal)CP(tBuGly)NR(Aib)(D-Asp)C   1.48 ± 0.7 ((β-Ala)-Sar₁₀-(SEQ ID NO: 221)) 251 (β-Ala)-Sar₁₀-H(D-Asp)VP-CP(1Nal)G(Aib)(HArg)(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C   1.93 ± 0.62 ((β-Ala)-Sar₁₀-(SEQ ID NO: 222)) 252 (β-Ala)-Sar₁₀-H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)NRPGC ((β-Ala)-Sar₁₀-   0.37 ± 0.18 (SEQ ID NO: 223)) 253 Ac-Sar₂-H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)PGC (Ac-Sar₂-(SEQ   0.85 ± 0.82 ID NO: 224)) 254 Ac-Sar₂-H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C (Ac-Sar₂-   0.74 ± 0.2 0.64 ± 0.28 (SEQ ID NO: 122)) 255 (β-Ala)-Sar₁₀-H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C ((β-   1.53 ± 0.58 Ala)-Sar₁₀-(SEQ ID NO: 122)) 256 Ac-(β-Ala)-Sar₁₀-H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C 0.41 n = 1 (Ac-(β-Ala)-Sar₁₀-(SEQ ID NO: 122)) 257 (β-Ala)-Sar₁₀-H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)PGC ((β-   1.07 ± 0.2 Ala)-Sar₁₀-(SEQ ID NO: 224)) 258 Ac-(β-Ala)-Sar₁₀-H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)GC (Ac-(β- 0.54 n = 1 Ala)-Sar₁₀-(SEQ ID NO: 123)) 259 (β-Ala)-Sar₁₀-H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)(Aib)(D-Asp)C   0.91 ± 0.14 ((β-Ala)-Sar₁₀-(SEQ ID NO: 225)) 260 Ac-(β-Ala)-Sar₁₀-H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)(D-Asp)C   0.75 ± 0.07 (Ac-(β-Ala)-Sar₁₀-(SEQ ID NO: 124)) 261 Ac-Sar₂-H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CPVNR(Aib)GC(Ac-Sar₂-(SEQ ID NO:   0.63 ± 0.43 226)) 262 Ac-Sar₂-H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)GC (Ac-Sar₂-(SEQ ID   0.71 ± 0.17 0.72 ± 0.31 NO: 123)) 263 (β-Ala)-Sar₁₀-H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)GC ((β-Ala)-   0.73 ± 0.26 Sar₁₀-(SEQ ID NO: 123)) 264 (β-Ala)-Sar₁₀-H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)(D-Asp)C ((β-   0.61 ± 0.31 Ala)-Sar₁₀-(SEQ ID NO: 124)) 265 (β-Ala)-Sar₁₀-H(D-Asp)VP-CP(1Nal)G(Aib)FWCP(tBuGly)N(HArg)P(D-Asp)C ((β-   1.55 ± 0.34 Ala)-Sar₁₀-(SEQ ID NO: 227)) 266 Ac-Sar₂-H(D-Asp)VP-CP(1Nal)G(Aib)FWCPVNR(Aib)GC (Ac-Sar₂-(SEQ ID NO: 228))    1.6 ± 0.63 267 Ac-Sar₂-H(D-Asp)VP-CPW(Aza-Gly)PFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 229))   0.66 ± 0.2 268 Ac-Sar₂-H(D-Asp)VP-CPWG(Aib)F(1Nal)CPVNR(Aib)GC (Ac-Sar₂-(SEQ ID NO: 230))   1.24 ± 0.46 269 (β-Ala)-Sar₁₀-H(D-Asp)VP-CPWG(Aib)F(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C ((β-   1.11 ± 0.08 Ala)-Sar₁₀-(SEQ ID NO: 231)) 270 Ac-Sar₂-H(D-Asp)VP-CPWG(Aib)FWCP(tBuGly)NR(Aib)GC (Ac-Sar₂-(SEQ ID NO:   1.52 ± 1.27 232)) 271 (β-Ala)-Sar₁₀-H(D-Asp)VP-CPWG(Aib)FWCP(tBuGly)N(HArg)P(D-Asp)C ((β-   3.66 ± 1.7 Ala)-Sar₁₀-(SEQ ID NO: 233)) 272 (β-Ala)-Sar₁₀-H(D-Asp)VP-CPWGP(HArg)WCP(tBuGly)N(HArg)P(D-Asp)C ((β-Ala)-   3.99 ± 0.13 Sar₁₀-(SEQ ID NO: 234)) 273 (β-Ala)-Sar₁₀-H(D-Asp)(C5g)P-C(Aib)(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)(D-Asp)C 1.5 n = 1 ((β-Ala)-Sar₁₀-(SEQ ID NO: 125)) 274 Ac-(β-Ala)-Sar₁₀-H(D-Asp)(C5g)P-C(Aib)(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)(D-   2.28 ± 0.69 Asp)C (Ac-(β-Ala)-Sar₁₀-(SEQ ID NO: 125)) 275 (β-Ala)-Sar₁₀-H(D-Asp)(C5g)P-C(Aib)WG(Aib)FWCP(tBuGly)NR(Aib)(D-Asp)C ((β-   15.9 ± 0.2 Ala)-Sar₁₀-(SEQ ID NO: 235)) 276 (β-Ala)-Sar₁₀-H(D-Asp)(C5g)P-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C   0.62 ± 0.27 ((β-Ala)-Sar₁₀-(SEQ ID NO: 126)) 277 Ac-(β-Ala)-Sar₁₀-H(D-Asp)(C5g)P-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)P(D-   0.53 ± 0.15 Asp)C (Ac-(β-Ala)-Sar₁₀-(SEQ ID NO: 126)) 278 (β-Ala)-Sar₁₀-H(D-Asp)(C5g)P-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)GC ((β-   0.46 ± 0.22 Ala)-Sar₁₀-(SEQ ID NO: 236)) 279 (β-Ala)-Sar₁₀-H(D-Asp)(C5g)T-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)GC ((β-   0.59 ± 0.28 Ala)-Sar₁₀-(SEQ ID NO: 237)) 280 (β-Ala)-Sar₁₀-H(D-Asp)(C5g)T-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C   0.64 ± 0.48 ((β-Ala)-Sar₁₀-(SEQ ID NO: 238)) 281 Ac-Sar₂-A(D-Asp)DVT-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 239))   5.78 ± 1.1 282 Ac-Sar₂-A(D-Asp)DVT-CP(1Nal)GPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 240))   0.87 ± 0.14 283 Ac-Sar₂-A(D-Asp)DVT-CP(1Nal)GPF(1Nal)CPVNRPGC (Ac-Sar₂-(SEQ ID NO: 241))   0.28 ± 0.08 284 Ac-Sar₂-A(D-Asp)DVT-CP(1Nal)G(Aib)FWCP(tBuGly)N(HArg)PGC (Ac-Sar₂-(SEQ ID    3.8 ± 0.77 NO: 242)) 285 Ac-Sar₂-H(D-Asp)VT-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)GC (Ac-Sar₂-(SEQ ID   1.21 ± 0.29 NO: 243))

TABLE 9 Biological Assay Data for TBMB Peptide Ligands of the Invention (Ala scan peptides, Competition Binding Assay) Ki, nM ± 95% Cl Bicycle Human EphA2 Compound Fluorescent Peptide Number Sequence Compound 66 Compound 18 25 Ac-CANDWLCSLGWTC (Ac-(SEQ ID NO: 23)) 328 n = 1 26 Ac-CMNDWLCALGWTC (Ac-(SEQ ID NO: 24))   71.6 ± 3.33 27 Ac-CMNDWLCSAGWTC (Ac-(SEQ ID NO: 25)) 356 n = 1 286 Sar₂-ACAWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 244))    886 ± 474.47 287 Sar₂-ACPAGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 245)) >11000 288 Sar₂-ACPWAPFWCPVNRPGC (Sar₂-(SEQ ID NO: 246)) >28000 >1000 289 Sar₂-ACPWGAFWCPVNRPGC (Sar₂-(SEQ ID NO: 247))   1102 ± 186.2 >1000 83 Sar₂-ACPWGPAWCPVNRPGC (Sar₂-(SEQ ID NO: 63))  287.5 ± 197.95 199 n = 1 290 Sar₂-ACPWGPFACPVNRPGC (Sar₂-(SEQ ID NO: 248)) >7000 291 Sar₂-ACPWGPFWCAVNRPGC (Sar₂-(SEQ ID NO: 249)) >6000 >1000 292 Sar₂-ACPWGPFWCPANRPGC (Sar₂-(SEQ ID NO: 250))  953.5 ± 59.78 101 Sar₂-ACPWGPFWCPVARPGC (Sar₂-(SEQ ID NO: 81))    265 ± 235.04 293 n = 1 293 Sar₂-ACPWGPFWCPVNAPGC (Sar₂-(SEQ ID NO: 251))    711 ± 581.64 104 Sar₂-ACPWGPFWCPVNRAGC (Sar₂-(SEQ ID NO: 84)) 193.67 ± 112.76 124 n = 1 111 Sar₂-ACPWGPFWCPVNRPAC (Sar₂-(SEQ ID NO: 91))  237.5 ± 164.92 108 n = 1 294 Sar₂-AC(D-Ala)WGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 252)) >4000 295 Sar₂-ACP(D-Ala)GPFWCPVNRPGC (Sar₂-(SEQ ID NO: 253)) >7000 296 Sar₂-ACPW(D-Ala)PFWCPVNRPGC (Sar₂-(SEQ ID NO: 254)) 1003 n = 1 297 Sar₂-ACPWG(D-Ala)FWCPVNRPGC (Sar₂-(SEQ ID NO: 255)) 1497 n = 1 298 Sar₂-ACPWGP(D-Ala)WCPVNRPGC (Sar₂-(SEQ ID NO: 256)) >6500 299 Sar₂-ACPWGPF(D-Ala)CPVNRPGC (Sar₂-(SEQ ID NO: 257)) >4000 300 Sar₂-ACPWGPFWC(D-Ala)VNRPGC (Sar₂-(SEQ ID NO: 258)) >1200 301 Sar₂-ACPWGPFWCP(D-Ala)NRPGC (Sar₂-(SEQ ID NO: 259)) >4000 102 Sar₂-ACPWGPFWCPV(D-Ala)RPGC (Sar₂-(SEQ ID NO: 82)) 311.67 ± 195.55    317 ± 168.56 302 Sar₂-ACPWGPFWCPVN(D-Ala)PGC (Sar₂-(SEQ ID NO: 260))   1410 ± 680.11 >1000 105 Sar₂-ACPWGPFWCPVNR(D-Ala)GC (Sar₂-(SEQ ID NO: 85)) 470.67 ± 221.53 677 n = 1 112 Sar₂-ACPWGPFWCPVNRP(D-Ala)C (Sar₂-(SEQ ID NO: 92)) 109.83 ± 66.19    89 ± 15.68 303 (β-Ala)-Sar₁₀-ACPWAPFWCAVNRPGC ((β-Ala)-Sar₁₀-(SEQ  >1000 ID NO: 261)) 304 4-(Pyridyl-2-disulfanyl)-4-RS-methylbutanoyl-(β-Ala)- >10000 Sar₁₀-ACPWAPFWCAVNRPGC (4-(Pyridyl-2-disulfanyl)-4- RS-methylbutanoyl-(β-Ala)-Sar₁₀-(SEQ ID NO: 261)) 173 Ac-Sar₂-A(D-Asp)VTCPWGPAWCPVNRPGC (Ac-Sar₂-(SEQ ID NO:   6.69 ± 3.49 152)) 305 (β-Ala)-Sar₁₀-H(D-Asp)VPCP(1Nal)A(Aib)F(1Nal)CA(tBuGly) >5000 NR(Aib)(D-Asp)C ((β-Ala)-Sar₁₀-(SEQ ID NO: 262)) 151 ADVHCPW(Ala)PFWCPVNRPGCA (SEQ ID NO: 130)  393.5 ± 206.78 152 ADVHCPWG(Ala)FWCPVNRPGCA (SEQ ID NO: 131)    1.8 ± 0.74 154 ADVHCPWGPFWC(D-Ala)VNRPGCA (SEQ ID NO: 133) 101.03 ± 33.68 155 ADVHCPWGPFWCPV(D-Ala)RPGCA (SEQ ID NO: 134) 2 ± 0.74 156 ADVHCPWGPFWCPVN(D-Ala)PGCA (SEQ ID NO: 135)  14.93 ± 2.3 306 DOTA-(β-Ala)-Sar₁₀-H(D-Asp)VPCP(1Nal)A(Aib)F(1Nal)CA >250 (tBuGly)NR(Aib)(D-Asp)C(DOTA-(β-Ala)-Sar₁₀-(SEQ ID NO: 262))

TABLE 10 Biological Assay Data for TBMB Peptide Ligands of the Invention (Ala scan peptides, Direct Binding Assay) Bicycle Compound K_(D), nM ± 95% Cl Number Sequence Human EphA2 307 AF488-(β-Ala)-Sar₁₀-ACPWAPFWCAVNRPGC (AF488-(β-Ala)-Sar₁₀- >1000 (SEQ ID NO: 261)) 308 AF488-(β-Ala)-Sar₁₀-H(D-Asp)VPCP(1Nal)A(Aib)F(1Nal)CA(tBuGly) NR(Aib)(D-Asp)C (AF488-(β-Ala)-Sar₁₀-(SEQ ID NO: 262)) >2000

TABLE 11 Biological Assay Data for TBMB Peptide Ligands of the Invention (BDC comp data with TBMB Scaffolds) BDC Ki, nM ± 95% Cl Human EphA2 Compound Bicycle General Fluorescent Peptide Number precursor Formula Compound 66 Compound 18 Compound 12 BDC-1 Compound 72 Formula (A) 41.3 n = 1 10.5 ± 0.98 78.1 n = 1 BDC-2 Compound 72 Formula (B) 22.23 ± 10.77 BDC-3 Compound 226 Formula (B) 0.38 ± 0.1  BDC-4 Compound 227 Formula (B) 1.72 ± 0.99 BDC-5 Compound 303 Formula (B) >1000 >1000

TABLE 12 Selectivity Data for Peptide Ligands of the Invention (Selectivity Direct Binding Assay) Bicycle Human rat & Compound mouse rat dog & mouse rat human mouse rat Number EphA2 EphA2 EphA2 EphA3 EphA3 EphA4 EphA4 EphB1 1 301 >2000 >5000 n = 1 12 55.5 n = 1 66 22 >2000 >5000 2268 n = 1 n = 1 67 51.8 ± 26.5 ± 61.43 ± >10000 >4000 >5000 >1000 6.27 10.69 27.05 144 2.56 1.25 4.39 166 >2500 1444 n = 1 n = 1 n = 1 n = 1 n = 1 145 3.8 ± 1 ± 4.99 ± 129.25 ± 297 ± 1497.2 ± 2535.33 ± 5421 0.55 0.4 1.02 13.3 71.24 1151.8 2325.76 n = 1 143 4.69 ± 1.38 ± 4.47 ± 259.5 ± 474 5990000 1000000 1090 4.15 1.36 3.09 8.82 n = 1 n = 1 n = 1 n = 1 140 4.55 ± 1.57 ± 8.7 ± 3691 >4000 >2500 >2000 >2000 1.04 0.46 3.23 n = 1 147 3.44 ± 1.15 ± 3.58 ± 82.5 ± 221 ± 7441983.67 ± 1002814.33 ± 1.31 0.33 0.89 20.12 61.76 8013305.7 1957206.91 141 6.5 ± 2.33 ± 13.33 ± >4000 137 237610 ± 1889 0.63 0.49 1.77 n = 1 384917.33 n = 1 308 >2000 >2000 >2000 >4000 >4000 >5000 >1000 139 0.8 ± 0.36 ± 2.17 ± >10000 >4000 >4000 >4000 >2000 0.54 0.08 0.76 68 23.4 15.9 31.9 >10000 >4000 >4000 >4000 >2000 n = 1 n = 1 n = 1 18 4.02 ± 1.72 3.57 >2000 >1000 1418 2 n = 1 n = 1 n = 1 23 386 146 7186 n = 1 human Bicycle human Carbonic Compound Human Human Human Human Factor anhydrase human Number EphB4 EphA7 EphA6 EphA1 XIIa 9 CD38 1 >1000 1640 n = 1 12 66 >4000 >1000 >3000 1178 n = 1 67 144 >4000 >1000 >3000 1712 n = 1 145 >4000 >1000 >3000 7260 n = 1 143 >4000 >1000 >3000 1283 n = 1 140 >4000 >1000 >3000 >1000 >8000 >10000 >10000 147 >4000 >1000 >3000 1218 n = 1 141 308 139 >4000 >1000 >2000 >2000 >8000 >10000 >10000 68 >4000 >1000 >3000 >2000 >8000 >10000 >10000 18 >4000 >1000 >3000 >2000 23 146 

The invention claimed is:
 1. A peptide ligand specific for EphA2 comprising a polypeptide comprising at least three cysteine residues, separated by at least two loop sequences, and an aromatic molecular scaffold which forms covalent bonds with the cysteine residues of the polypeptide such that at least two polypeptide loops are formed on the molecular scaffold, wherein the molecular scaffold is 1,3,5-tris(bromomethyl)benzene (TBMB), and wherein the polypeptide comprises an amino acid sequence selected from: ACMNDWLCSLGWTCA (SEQ ID NO: 1); ACMNDWLCSLGWTC (SEQ ID NO: 2); ACMNDWLCELGWTCA (SEQ ID NO: 3); ACTRQGIWCALGFEPCA (SEQ ID NO: 4); ACMNDWLCTLGWSCA (SEQ ID NO: 5); ACMNDWLCQLGWTCA (SEQ ID NO: 6); ACMNDWLCTLGWTCA (SEQ ID NO: 7); ACMNDWLCDLGWRCA (SEQ ID NO: 8); ACMNDWLCELGWSCA (SEQ ID NO: 9); ACRVSPEYCPFGPVWCAGAAA (SEQ ID NO: 10); ACPWGPAWCPVHGKTCA (SEQ ID NO: 11); ACPWGPAWCPVNRPGCA (SEQ ID NO: 12); ACPWGPAWCPVNRPGCAGAAA (SEQ ID NO: 13); ACPWGPMWCPVNRPGCA (SEQ ID NO: 14); ACPWGPNWCPVNRPGCA (SEQ ID NO: 15); AGEMACPWGPFWCPVNRPGCA (SEQ ID NO: 16); ADVTCPWGPFWCPVNRPGCA (SEQ ID NO: 17); ADVRTCPWGPFWCPVNRPGCA (SEQ ID NO: 18); ANDVTCPWGPFWCPVNRPGCA (SEQ ID NO: 19); ACVPQGIWCALQFEPCA (SEQ ID NO: 20); ACQKQGLWCALGFEPCA (SEQ ID NO: 21); ACLVNDDCFYMGLCA (SEQ ID NO: 22); CANDWLCSLGWTC (SEQ ID NO: 23); CMNDWLCALGWTC (SEQ ID NO: 24); CMNDWLCSAGWTC (SEQ ID NO: 25); ACMNDWLCQLGWKCA (SEQ ID NO: 26); ACTQNDWLCSLGWTCA (SEQ ID NO: 27); ACRNIPTMCPFGPVWCA (SEQ ID NO: 28); ACRVSPEYCPFGPVWCA (SEQ ID NO: 29); ACRVSPEYCPFGPTWCA (SEQ ID NO: 30); ACRVSPEYCPFGPSWCA (SEQ ID NO: 31); ACRVSPEYCPFGPEWCA (SEQ ID NO: 32); ACRVSPEYCPFGPYWCA (SEQ ID NO: 33); ACRVSPEYCPFGPLWCA (SEQ ID NO: 34); ACRVSPEYCPFGPDWCA (SEQ ID NO: 35); ACPWGPAWCPVRDTNCA (SEQ ID NO: 36); ACPWGPAWCPVNGARCA (SEQ ID NO: 37); ACPWGPAWCPVRNPCA (SEQ ID NO: 38); ACPWGPAWCPVSRVCA (SEQ ID NO: 39); ACPWGPAWCPVRSCA (SEQ ID NO: 40); ACPWGPAWCPVKPTCA (SEQ ID NO: 41); ACPWGPAWCPVNRNGCA (SEQ ID NO: 42); AVHIPCPWGPSWCPVNRPGCA (SEQ ID NO: 43); AEGLPCPWGPFWCPVNRPGCA (SEQ ID NO: 44); ADHACPWGPFWCPVNRPGCA (SEQ ID NO: 45); ADVHCPWGPFWCPVNRPGCA (SEQ ID NO: 46); AHDVPCPWGPFWCPVNRPGCA (SEQ ID NO: 47); ARDDPCPWGPFWCPVNRPGCA (SEQ ID NO: 48); ACTTGSIWCALQFEPCA (SEQ ID NO: 49); ACVPQGIWCALRYEPCA (SEQ ID NO: 50); ACPWGPFWCPVNRPGCA (SEQ ID NO: 51); ACPWGPFWCPVNRPGC (SEQ ID NO: 52); AC(HyP)WGPFWCPVNRPGC (SEQ ID NO: 53); AC(Aib)WGPFWCPVNRPGC (SEQ ID NO: 54); AC(4FlPro)WGPFWCPVNRPGC (SEQ ID NO: 55); ACP(1Nal)GPFWCPVNRPGC (SEQ ID NO: 56); ACP(2Nal)GPFWCPVNRPGC (SEQ ID NO: 57); ACPWG(Aze)FWCPVNRPGC (SEQ ID NO: 58); ACPWG(HyP)FWCPVNRPGC (SEQ ID NO: 59); ACPWG(Aib)FWCPVNRPGC (SEQ ID NO: 60); ACPWG(4FlPro)FWCPVNRPGC (SEQ ID NO: 61); ACPWG(Pip)FWCPVNRPGC (SEQ ID NO: 62); ACPWGPAWCPVNRPGC (SEQ ID NO: 63); ACPWGP(4Pal)WCPVNRPGC (SEQ ID NO: 64); ACPWGP(4BrPhe)WCPVNRPGC (SEQ ID NO: 65); ACPWGP(4MeOPhe)WCPVNRPGC (SEQ ID NO: 66); ACPWGP(HPhe)WCPVNRPGC (SEQ ID NO: 67); ACPWGP(4,4-BPA)WCPVNRPGC (SEQ ID NO: 68); ACPWGP(NO2Phe5)WCPVNRPGC (SEQ ID NO: 69); ACPWGP(3,4-DCPhe)WCPVNRPGC (SEQ ID NO: 70); ACPWGPYWCPVNRPGC (SEQ ID NO: 71); ACPWGP(3Pal)WCPVNRPGC (SEQ ID NO: 72); ACPWGP(Phg)WCPVNRPGC (SEQ ID NO: 73); ACPWGP(1Nal)WCPVNRPGC (SEQ ID NO: 74); ACPWGP(2Nal)WCPVNRPGC (SEQ ID NO: 75); ACPWGPF(1Nal)CPVNRPGC (SEQ ID NO: 76); ACPWGPFWC(Aze)VNRPGC (SEQ ID NO: 77); ACPWGPFWC(HyP)VNRPGC (SEQ ID NO: 78); ACPWGPFWC(4F1Pro)VNRPGC (SEQ ID NO: 79); ACPWGPFWCP(tBuGly)NRPGC (SEQ ID NO: 80); ACPWGPFWCPVARPGC (SEQ ID NO: 81); ACPWGPFWCPV(D-Ala)RPGC (SEQ ID NO: 82); ACPWGPFWCPVN(HArg)PGC (SEQ ID NO: 83); ACPWGPFWCPVNRAGC (SEQ ID NO: 84); ACPWGPFWCPVNR(D-Ala)GC (SEQ ID NO: 85); ACPWGPFWCPVNR(Aze)GC (SEQ ID NO: 86); ACPWGPFWCPVNR(HyP)GC (SEQ ID NO: 87); ACPWGPFWCPVNR(Pip)GC (SEQ ID NO: 88); ACPWGPFWCPVNR(4FlPro)GC (SEQ ID NO: 89); ACPWGPFWCPVNR(Aib)GC (SEQ ID NO: 90); ACPWGPFWCPVNRPAC (SEQ ID NO: 91); ACPWGPFWCPVNRP(D-Ala)C (SEQ ID NO: 92); AC(Aib)(1Nal2)GPFWCPVNRPGC (SEQ ID NO: 93); AC(Aib)WGPF(1Nal)CPVNRPGC (SEQ ID NO: 94); ACP(1Nal)GPFWCPV(D-Ala)RPGC (SEQ ID NO: 95); ACP(1Nal)GPFWCPVNRP(D-Ala)C (SEQ ID NO: 96); ACPWGPF(1Nal)CPV(D-Ala)RPGC (SEQ ID NO: 97); ACPWGPF(1Nal)CPVNRP(D-Ala)C (SEQ ID NO: 98); ACP(1Nal)G(Aib)FWCPVNRPGC (SEQ ID NO: 99); ACP(1Nal)GPF(1Nal)CPVNRPGC (SEQ ID NO: 100); ACP(1Nal)GPFWCP(tBuGly)NRPGC (SEQ ID NO: 101); ACP(1Nal)GPFWCPVN(HArg)PGC (SEQ ID NO: 102); ACPWG(Aib)F(1Nal)CPVNRPGC (SEQ ID NO: 103); AC(Aib)(1Nal)GPFWCPV(D-Ala)RPGC (SEQ ID NO: 104); AC(Aib)(1Nal)GPFWCPVNRP(D-Ala)C (SEQ ID NO: 105); ACP(1Nal)G(Aib)FWCP(tBuGly)NRPGC (SEQ ID NO: 106); ACP(1Nal)G(Aib)FWCPV(D-Ala)RPGC (SEQ ID NO: 107); ACP(1Nal)G(Aib)FWCPVNRP(D-Ala)C (SEQ ID NO: 108); ACP(1Nal)GPFWCP(tBuGly)(D-Ala)RPGC (SEQ ID NO: 109); ACP(1Nal)GPFWCP(tBuGly)N(HArg)PGC (SEQ ID NO: 110); ACP(1Nal)GPFWCP(tBuGly)NR(Aib)GC (SEQ ID NO: 111); ACP(1Nal)GPFWCP(tBuGly)NRP(D-Ala)C (SEQ ID NO: 112); ACP(1Nal)GPFWCPV(D-Ala)(HArg)PGC (SEQ ID NO: 113); ACP(1Nal)GPFWCPVN(HArg)P(D-Ala)C (SEQ ID NO: 114); AC(Aib)(1Nal)G(Aib)FWCPVNR(Aib)GC (SEQ ID NO: 115); ACP(1Nal)G(Aib)FWCP(tBuGly)N(HArg)PGC (SEQ ID NO: 116); ACP(1Nal)G(Aib)FWCP(tBuGly)NR(Aib)GC (SEQ ID NO: 117); ACP(1Nal)GPFWCP(tBuGly)N(HArg)(Aib)GC (SEQ ID NO: 118); H(D-Asp)VPCPWGPFWCPVNRPGCA (SEQ ID NO: 119); H(D-Asp)VP-C(Aib)(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C (SEQ ID NO: 120); H(D-Asp)VP-C(Aib)(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)(D-Asp)C (SEQ ID NO: 121); H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C (SEQ ID NO: 122); H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C (SEQ ID NO: 122); H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)GC (SEQ ID NO: 123); H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)(D-Asp)C (SEQ ID NO: 124); H(D-Asp)(C5g)P-C(Aib)(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)(D-Asp)C (SEQ ID NO: 125); H(D-Asp)(C5g)P-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C (SEQ ID NO: 126); ADVH-CPWGPFWCPVNRPGC (SEQ ID NO: 127); ADVH-CP(3,3-DPA)GPFWCPVNRPGCA (SEQ ID NO: 128); ADVH-CP(1Nal)GPFWCPVNRPGCA (SEQ ID NO: 129); ADVH-CPWAPFWCPVNRPGCA (SEQ ID NO: 130); ADVH-CPWGAFWCPVNRPGCA (SEQ ID NO: 131); ADVH-CPWG(Aib)FWCPVNRPGCA (SEQ ID NO: 132); ADVH-CPWGPFWCAPVNRPGCA (SEQ ID NO: 133); ADVH-CPWGPFWCPV(D-Ala)RPGCA (SEQ ID NO: 134); ADVH-CPWGPFWCPVN(D-Ala)PGCA (SEQ ID NO: 135); ADVT-CPWGPFWCPVNRPGC (SEQ ID NO: 136); A(D-Asp)VT-CPWGPFWCPVNRPGC (SEQ ID NO: 137); A(D-Asp)(D-Asp)T-CPWGPFWCPVNRPGC (SEQ ID NO: 138); A(D-Asp)(Cba)T-CPWGPFWCPVNRPGC (SEQ ID NO: 139); A(D-Asp)(Cpa)T-CPWGPFWCPVNRPGC (SEQ ID NO: 140); A(D-Asp)(Cpg)T-CPWGPFWCPVNRPGC (SEQ ID NO: 141); A(D-Asp)(C5g)VT-CPWGPFWCPVNRPGC (SEQ ID NO: 142); AD(tBuGly)T-CPWGPFWCPVNRPGC (SEQ ID NO: 143); A(D-Asp)VT-C(AC3C)WGPFWCPVNRPGC (SEQ ID NO: 144); A(D-Asp)VT-C(AC4C)WGPFWCPVNRPGC (SEQ ID NO: 145); A(D-Asp)VT-C(AC5C)WGPFWCPVNRPGC (SEQ ID NO: 146); A(D-Asp)VT-C(4BenzyPro)WGPFWCPVNRPGC (SEQ ID NO: 147); A(D-Asp)VT-C(4PhenyPro)WGPFWCPVNRPGC (SEQ ID NO: 148); A(D-Asp)VT-CP(1Nal)GPFWCPVNRPGC (SEQ ID NO: 149); A(D-Asp)VT-CPWGP(HArg)WCPVNRPGC (SEQ ID NO: 150); A(D-Asp)VT-CPWGPNWCPVNRPGC (SEQ ID NO: 151); A(D-Asp)VT-CPWGPAWCPVNRPGC (SEQ ID NO: 152); A(D-Asp)VT-CPWGPFWCPLNRPGC (SEQ ID NO: 153); A(D-Asp)VT-CPWGPFWCPVNRP(D-Asp)C (SEQ ID NO: 154); A(D-Asp)VT-CPWGPFWCPVN(HArg)P(D-Asp)C (SEQ ID NO: 155); A(D-Asp)VT-CPWGPFWCPVNR(Aib)(D-Asp)C (SEQ ID NO: 156); A(D-Asp)VT-CP(1Nal)G(Aib)FWCPVNR(Aib)GC (SEQ ID NO: 157); A(D-Asp)VT-CPWG(Aib)F(1Nal)CPVNR(Aib)GC (SEQ ID NO: 158); A(D-Asp)VT-CPWG(Aib)FWCP(tBuGly)NR(Aib)GC (SEQ ID NO: 159); A(D-Asp)VT-CP(1Nal)G(Aib)F(1Nal)CPVNR(Aib)GC (SEQ ID NO: 160); A(D-Asp)VT-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)PGC (SEQ ID NO: 161); A(D-Asp)VT-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)GC (SEQ ID NO: 162); A(D-Asp)VT-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C (SEQ ID NO: 163); (D-Asp)VT-CPWGPFWCPVNRPGC (SEQ ID NO: 164); (D-Asp)VT-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)GC (SEQ ID NO: 165); AHDVP-CP(1Nal)GPFWCPVNRPGCA (SEQ ID NO: 166); AHDVP-CP(1Nal)GPFWCPVNRPGC (SEQ ID NO: 167); AHDVP-CPWGPF(1Nal)CPVNRPGC (SEQ ID NO: 168); AHDVP-CP(1Nal)GPFWCP(tBuGly)NRPGC (SEQ ID NO: 169); AHDVP-CP(1Nal)G(Aib)FWCP(tBuGly)N(HArg)PGC (SEQ ID NO: 170); AHDVP-CP(1Nal)G(Aib)FWCP(tBuGly)NR(Aib)GC (SEQ ID NO: 171); AHDVP-CPWGPFWCPVNRPGC (SEQ ID NO: 172); (D-Ala)HDVP-CPWGPFWCPVNRPGC (SEQ ID NO: 173); AADVP-CPWGPFWCPVNRPGC (SEQ ID NO: 174); A(D-His)DVP-CPWGPFWCPVNRPGC (SEQ ID NO: 175); A(D-His)DVCPWGPFWCPVNRPGC (SEQ ID NO: 176); A(D-Ala)DVP-CPWGPFWCPVNRPGC (SEQ ID NO: 177); A(D-Asp)DVP-CPWGPFWCPVNRPGC (SEQ ID NO: 178); A(Thi)DVP-CPWGPFWCPVNRPGC (SEQ ID NO: 179); A(ThiAz)DVP-CPWGPFWCPVNRPGC (SEQ ID NO: 180); A(2FuAla)DVP-CPWGPFWCPVNRPGC (SEQ ID NO: 181); A(D-His)D(tBuGly)P-CPWGPFWCPVNRPGC (SEQ ID NO: 182); AHAVP-CPWGPFWCPVNRPGC (SEQ ID NO: 183); AH(D-Ala)VP-CPWGPFWCPVNRPGC (SEQ ID NO: 184); AHEVP-CPWGPFWCPVNRPGC (SEQ ID NO: 185); AH(D-Glu)VP-CPWGPFWCPVNRPGC (SEQ ID NO: 186); AH(D-Asp)VP-CPWGPFWCPVNRPGC (SEQ ID NO: 187); AH(D-Asp)(tBuGly)P-CPWGPFWCPVNRPGC (SEQ ID NO: 188); AH(D-Asp)V(Sar)-CPWGPFWCPVNRPGC (SEQ ID NO: 189); AH(D-Asp)V(Aib)-CPWGPFWCPVNRPGC (SEQ ID NO: 190); AHDAP-CPWGPFWCPVNRPGC (SEQ ID NO: 191); AHD(D-Ala)P-CPWGPFWCPVNRPGC (SEQ ID NO: 192); AHD(Aib)P-CPWGPFWCPVNRPGC (SEQ ID NO: 193); AHD(tBuGly)P-CPWGPFWCPVNRPGC (SEQ ID NO: 194); AHDVA-CPWGPFWCPVNRPGC (SEQ ID NO: 195); AHDV(D-Ala)-CPWGPFWCPVNRPGC (SEQ ID NO: 196); AHDV(Aib)-CPWGPFWCPVNRPGC (SEQ ID NO: 197); AHDV(Aze)-CPWGPFWCPVNRPGC (SEQ ID NO: 198); AHDV(Pip)-CPWGPFWCPVNRPGC [(SEQ ID NO: 199); HDVP-CPWGPFWCPVNRPGC (SEQ ID NO: 200); (D-His)DVP-CPWGPFWCPVNRPGC (SEQ ID NO: 201); H(D-Asp)VP-CPWGPFWCPVNRPGC (SEQ ID NO: 202); AH(D-Asp)VP-CP(1Nal)GPFWCP(tBuGly)N(HArg)PGC (SEQ ID NO: 203); A(D-His)DVP-CPWGP(ΨAla)WCPVNRPGC (SEQ ID NO: 204); A(D-His)DVP-CPWGPFWCP(HArg)NRPGC (SEQ ID NO: 205); A(D-His)DVP-CP(1Nal)GPFWCP(tBuGly)N(HArg)PGC (SEQ ID NO: 206); A(D-His)DVP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)PGC (SEQ ID NO: 207); A(D-His)DVP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)GC (SEQ ID NO: 208); A(D-His)DVP-CPWG(Aib)FWCP(tBuGly)NR(Aib)GC (SEQ ID NO: 209); H(D-Asp)VP-C(Aib)(1Nal)G(Aib)F(1Nal)CP(tBuGly)NRPGC (SEQ ID NO: 210); H(D-Asp)VP-C(Aib)(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)PGC (SEQ ID NO: 211); H(D-Asp)VP-C(Aib)(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)(Aib)(D-Asp)C (SEQ ID NO: 212); H(D-Asp)VP-C(Aib)(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)GC (SEQ ID NO: 213); (β-Ala)-Sar₁₀-H(D-Asp)VP-C(Aib)(1Nal)G(Aib)FWCP(tBuGly)N(HArg)P(D-Asp)C ((β-Ala)-Sar₁₀-(SEQ ID NO: 214)) H(D-Asp)VP-C(Aib)(1Nal)GP(HArg)(1Nal)CP(tBuGly)NR(Aib)GC (SEQ ID NO: 215); H(D-Asp)VP-C(Aib)(1Nal)GP(HArg)(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C (SEQ ID NO: 216); H(D-Asp)VP-C(Aib)WGP(HArg)(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C (SEQ ID NO: 217); H(D-Asp)VP-C(Aib)WGP(HArg)WCP(tBuGly)N(HArg)P(D-Asp)C (SEQ ID NO: 218); H(D-Asp)VP-C(D-Ala)(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C (SEQ ID NO: 219); H(D-Asp)VP-CP(1Nal)G(Aib)(HArg)(1Nal)CP(tBuGly)NR(Aib)GC (SEQ ID NO: 220); H(D-Asp)VP-CP(1Nal)G(Aib)(HArg)(1Nal)CP(tBuGly)NR(Aib)(D-Asp)C (SEQ ID NO: 221); H(D-Asp)VP-CP(1Nal)G(Aib)(HArg)(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C (SEQ ID NO: 222); H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)NRPGC (SEQ ID NO: 223); H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)PGC (SEQ ID NO: 224); H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)(Aib)(D-Asp)C (SEQ ID NO: 225); H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CPVNR(Aib)GC (SEQ ID NO: 226); H(D-Asp)VP-CP(1Nal)G(Aib)FWCP(tBuGly)N(HArg)P(D-Asp)C (SEQ ID NO: 227); H(D-Asp)VP-CP(1Nal)G(Aib)FWCPVNR(Aib)GC (SEQ ID NO: 228); H(D-Asp)VP-CPW(Aza-Gly)PFWCPVNRPGC (SEQ ID NO: 229); H(D-Asp)VP-CPWG(Aib)F(1Nal)CPVNR(Aib)GC (SEQ ID NO: 230); H(D-Asp)VP-CPWG(Aib)F(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C (SEQ ID NO: 231); H(D-Asp)VP-CPWG(Aib)FWCP(tBuGly)NR(Aib)GC (SEQ ID NO: 232); H(D-Asp)VP-CPWG(Aib)FWCP(tBuGly)N(HArg)P(D-Asp)C (SEQ ID NO: 233); H(D-Asp)VP-CPWGP(HArg)WCP(tBuGly)N(HArg)P(D-Asp)C (SEQ ID NO: 234); H(D-Asp)(C5g)P-C(Aib)WG(Aib)FWCP(tBuGly)NR(Aib)(D-Asp)C (SEQ ID NO: 235); H(D-Asp)(C5g)P-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)GC (SEQ ID NO: 236); H(D-Asp)(C5g)T-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)GC (SEQ ID NO: 237); H(D-Asp)(C5g)T-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C (SEQ ID NO: 238); A(D-Asp)DVT-CPWGPFWCPVNRPGC (SEQ ID NO: 239); A(D-Asp)DVT-CP(1Nal)GPFWCPVNRPGC (SEQ ID NO: 240); A(D-Asp)DVT-CP(1Nal)GPF(1Nal)CPVNRPGC (SEQ ID NO: 241); A(D-Asp)DVT-CP(1Nal)G(Aib)FWCP(tBuGly)N(HArg)PGC (SEQ ID NO: 242); H(D-Asp)VT-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)GC (SEQ ID NO: 243); ACAWGPFWCPVNRPGC (SEQ ID NO: 244); ACPAGPFWCPVNRPGC (SEQ ID NO: 245); ACPWAPFWCPVNRPGC (SEQ ID NO: 246); ACPWGAFWCPVNRPGC (SEQ ID NO: 247); ACPWGPFACPVNRPGC (SEQ ID NO: 248); ACPWGPFWCAVNRPGC (SEQ ID NO: 249); ACPWGPFWCPANRPGC (SEQ ID NO: 250); ACPWGPFWCPVNAPGC (SEQ ID NO: 251); AC(D-Ala)WGPFWCPVNRPGC (SEQ ID NO: 252); ACP(D-Ala)GPFWCPVNRPGC (SEQ ID NO: 253); ACPW(D-Ala)PFWCPVNRPGC (SEQ ID NO: 254); ACPWG(D-Ala)FWCPVNRPGC (SEQ ID NO: 255); ACPWGP(D-Ala)WCPVNRPGC (SEQ ID NO: 256); ACPWGPF(D-Ala)CPVNRPGC (SEQ ID NO: 257); ACPWGPFWC(D-Ala)VNRPGC (SEQ ID NO: 258); ACPWGPFWCP(D-Ala)NRPGC (SEQ ID NO: 259); ACPWGPFWCPVN(D-Ala)PGC (SEQ ID NO: 260); ACPWAPFWCAVNRPGC (SEQ ID NO: 261); and H(D-Asp)VPCP(1Nal)A(Aib)F(1Nal)CA(tBuGly)NR(Aib)(D-Asp)C (SEQ ID NO: 262); or a pharmaceutically acceptable salt thereof.
 2. The peptide ligand as defined in claim 1, wherein the peptide ligand is the free acid or a pharmaceutically acceptable salt selected from a sodium salt, a potassium salt, a calcium salt, and an ammonium salt.
 3. The peptide ligand as defined in claim 1, wherein the EphA2 is human EphA2.
 4. A pharmaceutical composition which comprises the peptide ligand as defined in claim 1, or a pharmaceutically acceptable salt thereof, in combination with one or more pharmaceutically acceptable excipients.
 5. A peptide ligand specific for EphA2 comprising a polypeptide comprising at least three cysteine residues, separated by at least two loop sequences, and an aromatic molecular scaffold which forms covalent bonds with the cysteine residues of the polypeptide such that at least two polypeptide loops are formed on the molecular scaffold, wherein the molecular scaffold is 1,3,5-tris(bromomethyl)benzene (TBMB), and wherein the polypeptide comprises an amino acid sequence selected from: Ac-CANDWLCSLGWTC (Ac-(SEQ ID NO: 23)); Ac-CMNDWLCALGWTC (Ac-(SEQ ID NO: 24)); Ac-CMNDWLCSAGWTC (Ac-(SEQ ID NO: 25)); Sar₂-ACPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 52)); Ac-Sar₂-ACPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 52)); (β-Ala)-Sar₁₀-ACPWGPFWCPVNRPGC ((β-Ala)-Sar₁₀-(SEQ ID NO: 52)); Sar₂-AC(HyP)WGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 53)); Sar₂-AC(Aib)WGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 54)); Sar₂-AC(4FlPro)WGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 55)); Sar₂-ACP(1Nal)GPFWCPVNRPGC (Sar₂-(SEQ ID NO: 56)); Sar₂-ACP(2Nal)GPFWCPVNRPGC (Sar₂-(SEQ ID NO: 57)); Sar₂-ACPWG(Aze)FWCPVNRPGC (Sar₂-(SEQ ID NO: 58)); Sar₂-ACPWG(HyP)FWCPVNRPGC (Sar₂-(SEQ ID NO: 59)); Sar₂-ACPWG(Aib)FWCPVNRPGC (Sar₂-(SEQ ID NO: 60)); Sar₂-ACPWG(4FlPro)FWCPVNRPGC (Sar₂-(SEQ ID NO: 61)); Sar₂-ACPWG(Pip)FWCPVNRPGC (Sar₂-(SEQ ID NO: 62)); Sar₂-ACPWGPAWCPVNRPGC (Sar₂-(SEQ ID NO: 63)); Sar₂-ACPWGP(4Pal)WCPVNRPGC (Sar₂-(SEQ ID NO: 64)); Sar₂-ACPWGP(4BrPhe)WCPVNRPGC (Sar₂-(SEQ ID NO: 65)); Sar₂-ACPWGP(4MeOPhe)WCPVNRPGC (Sar₂-(SEQ ID NO: 66)); Sar₂-ACPWGP(HPhe)WCPVNRPGC (Sar₂-(SEQ ID NO: 67)); Sar₂-ACPWGP(4,4-BPA)WCPVNRPGC (Sar₂-(SEQ ID NO: 68)); Sar₂-ACPWGP(NO2Phe5)WCPVNRPGC (Sar₂-(SEQ ID NO: 69)); Sar₂-ACPWGP(3,4-DCPhe)WCPVNRPGC (Sar₂-(SEQ ID NO: 70)); Sar₂-ACPWGPYWCPVNRPGC (Sar₂-(SEQ ID NO: 71)); Sar₂-ACPWGP(3Pal)WCPVNRPGC (Sar₂-(SEQ ID NO: 72)); Sar₂-ACPWGP(Phg)WCPVNRPGC (Sar₂-(SEQ ID NO: 73)); Sar₂-ACPWGP(1Nal)WCPVNRPGC (Sar₂-(SEQ ID NO: 74)); Sar₂-ACPWGP(2Nal)WCPVNRPGC (Sar₂-(SEQ ID NO: 75)); Sar₂-ACPWGPF(1Nal)CPVNRPGC (Sar₂-(SEQ ID NO: 76)); Sar₂-ACPWGPFWC(Aze)VNRPGC (Sar₂-(SEQ ID NO: 77)); Sar₂-ACPWGPFWC(HyP)VNRPGC (Sar₂-(SEQ ID NO: 78)); Sar₂-ACPWGPFWC(4FlPro)VNRPGC (Sar₂-(SEQ ID NO: 79)); Sar₂-ACPWGPFWCP(tBuGly)NRPGC (Sar₂-(SEQ ID NO: 80)); Sar₂-ACPWGPFWCPVARPGC (Sar₂-(SEQ ID NO: 81)); Sar₂-ACPWGPFWCPV(D-Ala)RPGC (Sar₂-(SEQ ID NO: 82)); Sar₂-ACPWGPFWCPVN(HArg)PGC (Sar₂-(SEQ ID NO: 83)); Sar₂-ACPWGPFWCPVNRAGC (Sar₂-(SEQ ID NO: 84)); Sar₂-ACPWGPFWCPVNR(D-Ala)GC (Sar₂-(SEQ ID NO: 85)); Sar₂-ACPWGPFWCPVNR(Aze)GC (Sar₂-(SEQ ID NO: 86)); Sar₂-ACPWGPFWCPVNR(HyP)GC (Sar₂-(SEQ ID NO: 87)); Sar₂-ACPWGPFWCPVNR(Pip)GC (Sar₂-(SEQ ID NO: 88)); Sar₂-ACPWGPFWCPVNR(4FlPro)GC (Sar₂-(SEQ ID NO: 89)); Sar₂-ACPWGPFWCPVNR(Aib)GC (Sar₂-(SEQ ID NO: 90)); Sar₂-ACPWGPFWCPVNRPAC (Sar₂-(SEQ ID NO: 91)); Sar₂-ACPWGPFWCPVNRP(D-Ala)C (Sar₂-(SEQ ID NO: 92)); Sar₂-AC(Aib)(1Nal2)GPFWCPVNRPGC (Sar₂-(SEQ ID NO: 93)); Sar₂-AC(Aib)WGPF(1Nal)CPVNRPGC (Sar₂-(SEQ ID NO: 94)); Sar₂-ACP(1Nal)GPFWCPV(D-Ala)RPGC (Sar₂-(SEQ ID NO: 95)); Sar₂-ACP(1Nal)GPFWCPVNRP(D-Ala)C (Sar₂-(SEQ ID NO: 96)); Sar₂-ACPWGPF(1Nal)CPV(D-Ala)RPGC (Sar₂-(SEQ ID NO: 97)); Sar₂-ACPWGPF(1Nal)CPVNRP(D-Ala)C (Sar₂-(SEQ ID NO: 98)); Sar₂-ACP(1Nal)G(Aib)FWCPVNRPGC (Sar₂-(SEQ ID NO: 99)); Sar₂-ACP(1Nal)GPF(1Nal)CPVNRPGC (Sar₂-(SEQ ID NO: 100)); Sar₂-ACP(1Nal)GPFWCP(tBuGly)NRPGC (Sar₂-(SEQ ID NO: 101)); Sar₂-ACP(1Nal)GPFWCPVN(HArg)PGC (Sar₂-(SEQ ID NO: 102)); Sar₂-ACPWG(Aib)F(1Nal)CPVNRPGC (Sar₂-(SEQ ID NO: 103)); Sar₂-AC(Aib)(1Nal)GPFWCPV(D-Ala)RPGC (Sar₂-(SEQ ID NO: 104)); Sar₂-AC(Aib)(1Nal)GPFWCPVNRP(D-Ala)C (Sar₂-(SEQ ID NO: 105)); Sar₂-ACP(1Nal)G(Aib)FWCP(tBuGly)NRPGC (Sar₂-(SEQ ID NO: 106)); Sar₂-ACP(1Nal)G(Aib)FWCPV(D-Ala)RPGC (Sar₂-(SEQ ID NO: 107)); Sar₂-ACP(1Nal)G(Aib)FWCPVNRP(D-Ala)C (Sar₂-(SEQ ID NO: 108)); Sar₂-ACP(1Nal)GPFWCP(tBuGly)(D-Ala)RPGC (Sar₂-(SEQ ID NO: 109)); Sar₂-ACP(1Nal)GPFWCP(tBuGly)N(HArg)PGC (Sar₂-(SEQ ID NO: 110)); Sar₂-ACP(1Nal)GPFWCP(tBuGly)NR(Aib)GC (Sar₂-(SEQ ID NO: 111)); Sar₂-ACP(1Nal)GPFWCP(tBuGly)NRP(D-Ala)C (Sar₂-(SEQ ID NO: 112)); Sar₂-ACP(1Nal)GPFWCPV(D-Ala)(HArg)PGC (Sar₂-(SEQ ID NO: 113)); Sar₂-ACP(1Nal)GPFWCPVN(HArg)P(D-Ala)C (Sar₂-(SEQ ID NO: 114)); Sar₂-AC(Aib)(1Nal)G(Aib)FWCPVNR(Aib)GC (Sar₂-(SEQ ID NO: 115)); Sar₂-ACP(1Nal)G(Aib)FWCP(tBuGly)N(HArg)PGC (Sar₂-(SEQ ID NO: 116)); Sar₂-ACP(1Nal)G(Aib)FWCP(tBuGly)NR(Aib)GC (Sar₂-(SEQ ID NO: 117)); Sar₂-ACP(1Nal)GPFWCP(tBuGly)N(HArg)(Aib)GC (Sar₂-(SEQ ID NO: 118)); Ac-Sar₂-ADVH-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 127)); Ac-Sar₂-ADVT-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 136)); Ac-Sar₂-A(D-Asp)VT-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 137)); Ac-Sar₂-A(D-Asp)(D-Asp)T-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 138)); Ac-Sar₂-A(D-Asp)(Cba)T-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 139)); Ac-Sar₂-A(D-Asp)(Cpa)T-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 140)); Ac-Sar₂-A(D-Asp)(Cpg)T-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 141)); Ac-Sar₂-A(D-Asp)(C5g)VT-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 142)); Ac-Sar₂-AD(tBuGly)T-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 143)); Ac-Sar₂-A(D-Asp)VT-C(AC3C)WGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 144)); Ac-Sar₂-A(D-Asp)VT-C(AC4C)WGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 145)); Ac-Sar₂-A(D-Asp)VT-C(AC5C)WGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 146)); Ac-Sar₂-A(D-Asp)VT-C(4BenzyPro)WGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 147)); Ac-Sar₂-A(D-Asp)VT-C(4PhenyPro)WGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 148)); Ac-Sar₂-A(D-Asp)VT-CP(1Nal)GPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 149)); Ac-Sar₂-A(D-Asp)VT-CPWGP(HArg)WCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 150)); Ac-Sar₂-A(D-Asp)VT-CPWGPNWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 151)); Ac-Sar₂-A(D-Asp)VT-CPWGPAWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 152)); Ac-Sar₂-A(D-Asp)VT-CPWGPFWCPLNRPGC (Ac-Sar₂-(SEQ ID NO: 153)); Ac-Sar₂-A(D-Asp)VT-CPWGPFWCPVNRP(D-Asp)C (Ac-Sar₂-(SEQ ID NO: 154)); Ac-Sar₂-A(D-Asp)VT-CPWGPFWCPVN(HArg)P(D-Asp)C (Ac-Sar₂-(SEQ ID NO: 155)); Ac-Sar₂-A(D-Asp)VT-CPWGPFWCPVNR(Aib)(D-Asp)C (Ac-Sar₂-(SEQ ID NO: 156)); Ac-Sar₂-A(D-Asp)VT-CP(1Nal)G(Aib)FWCPVNR(Aib)GC (Ac-Sar₂-(SEQ ID NO: 157)); Ac-Sar₂-A(D-Asp)VT-CPWG(Aib)F(1Nal)CPVNR(Aib)GC (Ac-Sar₂-(SEQ ID NO: 158)); Ac-Sar₂-A(D-Asp)VT-CPWG(Aib)FWCP(tBuGly)NR(Aib)GC (Ac-Sar₂-(SEQ ID NO: 159)); Ac-Sar₂-A(D-Asp)VT-CP(1Nal)G(Aib)F(1Nal)CPVNR(Aib)GC (Ac-Sar₂-(SEQ ID NO: 160)); Ac-Sar₂-A(D-Asp)VT-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)PGC (Ac-Sar₂-(SEQ ID NO: 161)); Ac-Sar₂-A(D-Asp)VT-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)GC (Ac-Sar₂-(SEQ ID NO: 162)); Ac-Sar₂-A(D-Asp)VT-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C (Ac-Sar₂-(SEQ ID NO: 163)); Ac-Sar₂-AHDVP-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 172); Ac-Sar₂-(D-Ala)HDVP-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 173); Ac-Sar₂-AADVP-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 174); Ac-Sar₂-A(D-His)DVP-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 175); Sar₂-A(D-His)DVP-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 175); Ac-Sar₂-A(D-His)DVCPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 176); Sar₂-A(D-Ala)DVP-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 177); Ac-Sar₂-A(D-Asp)DVP-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 178); Sar₂-A(Thi)DVP-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 179); Sar₂-A(ThiAz)DVP-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 180); Sar₂-A(2FuAla)DVP-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 181); Ac-Sar₂-A(D-His)D(tBuGly)P-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 182); Sar₂-AHAVP-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 183); Sar₂-AH(D-Ala)VP-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 184); Sar₂-AHEVP-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 185); Sar₂-AH(D-Glu)VP-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 186); Sar₂-AH(D-Asp)VP-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 187); Ac-Sar₂-AH(D-Asp)VP-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 187); Ac-Sar₂-AH(D-Asp)(tBuGly)P-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 188); Ac-Sar₂-AH(D-Asp)V(Sar)-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 189); Ac-Sar₂-AH(D-Asp)V(Aib)-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 190); Sar₂-AHDAP-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 191); Sar₂-AHD(D-Ala)P-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 192); Sar₂-AHD(Aib)P-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 193); Sar₂-AHD(tBuGly)P-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 194); Sar₂-AHDVA-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 195); Sar₂-AHDV(D-Ala)-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 196); Sar₂-AHDV(Aib)-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 197); Sar₂-AHDV(Aze)-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 198); Sar₂-AHDV(Pip)-CPWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 199); (β-Ala)-Sar₁₀-HDVP-CPWGPFWCPVNRPGC ((β-Ala)-Sar₁₀-(SEQ ID NO: 200)); Ac-Sar₂-(D-His)DVP-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 201)); Ac-Sar₂-H(D-Asp)VP-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 202)); (β-Ala)-Sar₁₀-H(D-Asp)VP-CPWGPFWCPVNRPGC (β-Ala)-Sar₁₀-(SEQ ID NO: 202)); Ac-Sar₂-AH(D-Asp)VP-CP(1Nal)GPFWCP(tBuGly)N(HArg)PGC (Ac-Sar₂-(SEQ ID NO: 203)); Ac-Sar₂-A(D-His)DVP-CPWGP(ΨAla)WCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 204)); Ac-Sar₂-A(D-His)DVP-CPWGPFWCP(HArg)NRPGC (Ac-Sar₂-(SEQ ID NO: 205)); Ac-Sar₂-A(D-His)DVP-CP(1Nal)GPFWCP(tBuGly)N(HArg)PGC (Ac-Sar₂-(SEQ ID NO: 206)); Ac-Sar₂-A(D-His)DVP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)PGC (Ac-Sar₂-(SEQ ID NO: 207)); Ac-Sar₂-A(D-His)DVP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)GC (Ac-Sar₂-(SEQ ID NO: 208)); Ac-Sar₂-A(D-His)DVP-CPWG(Aib)FWCP(tBuGly)NR(Aib)GC (Ac-Sar₂-(SEQ ID NO: 209)); (β-Ala)-Sar₁₀-H(D-Asp)VP-C(Aib)(1Nal)G(Aib)F(1Nal)CP(tBuGly)NRPGC ((β-Ala)-Sar₁₀-(SEQ ID NO: 210)); (β-Ala)-Sar₁₀-H(D-Asp)VP-C(Aib)(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)PGC ((β-Ala)-Sar₁₀-(SEQ ID NO: 211)); (β-Ala)-Sar₁₀-H(D-Asp)VP-C(Aib)(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C ((β-Ala)-Sar₁₀-(SEQ ID NO: 120)); Ac-(β-Ala)-Sar₁₀-H(D-Asp)VP-C(Aib)(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C (Ac-(β-Ala)-Sar₁₀-(SEQ ID NO: 120)); (β-Ala)-Sar₁₀-H(D-Asp)VP-C(Aib)(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)(Aib)(D-Asp)C ((β-Ala)-Sar₁₀-(SEQ ID NO: 212)); (β-Ala)-Sar₁₀-H(D-Asp)VP-C(Aib)(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)GC ((β-Ala)-Sar₁₀-(SEQ ID NO: 213)); (β-Ala)-Sar₁₀-H(D-Asp)VP-C(Aib)(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)(D-Asp)C ((β-Ala)-Sar₁₀-(SEQ ID NO: 121)); Ac-(β-Ala)-Sar₁₀-H(D-Asp)VP-C(Aib)(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)(D-Asp)C (Ac-(β-Ala)-Sar₁₀-(SEQ ID NO: 121)); (β-Ala)-Sar₁₀-H(D-Asp)VP-C(Aib)(1Nal)G(Aib)FWCP(tBuGly)N(HArg)P(D-Asp)C ((β-Ala)-Sar₁₀-(SEQ ID NO: 214)); (β-Ala)-Sar₁₀-H(D-Asp)VP-C(Aib)(1Nal)GP(HArg)(1Nal)CP(tBuGly)NR(Aib)GC ((β-Ala)-Sar₁₀-(SEQ ID NO: 215)); (β-Ala)-Sar₁₀-H(D-Asp)VP-C(Aib)(1Nal)GP(HArg)(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C ((β-Ala)-Sar₁₀-(SEQ ID NO: 216)); (β-Ala)-Sar₁₀-H(D-Asp)VP-C(Aib)WGP(HArg)(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C ((β-Ala)-Sar₁₀-(SEQ ID NO: 217)); (β-Ala)-Sar₁₀-H(D-Asp)VP-C(Aib)WGP(HArg)WCP(tBuGly)N(HArg)P(D-Asp)C ((β-Ala)-Sar₁₀-(SEQ ID NO: 218)); (β-Ala)-Sar₁₀-H(D-Asp)VP-C(Aib)WGP(HArg)WCP(tBuGly)N(HArg)P(D-Asp)C ((β-Ala)-Sar₁₀-(SEQ ID NO: 218)); (β-Ala)-Sar₁₀-H(D-Asp)VP-C(D-Ala)(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C ((β-Ala)-Sar₁₀-(SEQ ID NO: 219)); (β-Ala)-Sar₁₀-H(D-Asp)VP-CP(1Nal)G(Aib)(HArg)(1Nal)CP(tBuGly)NR(Aib)GC ((β-Ala)-Sar₁₀-(SEQ ID NO: 220)); (β-Ala)-Sar₁₀-H(D-Asp)VP-CP(1Nal)G(Aib)(HArg)(1Nal)CP(tBuGly)NR(Aib)(D-Asp)C ((β-Ala)-Sar₁₀-(SEQ ID NO: 221)); (β-Ala)-Sar₁₀-H(D-Asp)VP-CP(1Nal)G(Aib)(HArg)(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C ((β-Ala)-Sar₁₀-(SEQ ID NO: 222)); (β-Ala)-Sar₁₀-H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)NRPGC ((β-Ala)-Sar₁₀-(SEQ ID NO: 223)); Ac-Sar₂-H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)PGC (Ac-Sar₂-(SEQ ID NO: 224)); Ac-Sar₂-H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C (Ac-Sar₂-(SEQ ID NO: 122)); (β-Ala)-Sar₁₀-H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C ((β-Ala)-Sar₁₀-(SEQ ID NO: 122)); Ac-(β-Ala)-Sar₁₀-H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C (Ac-(β-Ala)-Sar₁₀-(SEQ ID NO: 122)); (β-Ala)-Sar₁₀-H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)PGC ((β-Ala)-Sar₁₀-(SEQ ID NO: 224)); Ac-(β-Ala)-Sar₁₀-H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)GC (Ac-(β-Ala)-Sar₁₀-(SEQ ID NO: 123)); (β-Ala)-Sar₁₀-H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)(Aib)(D-Asp)C ((β-Ala)-Sar₁₀-(SEQ ID NO: 225)); Ac-(β-Ala)-Sar₁₀-H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)(D-Asp)C (Ac-(β-Ala)-Sar₁₀-(SEQ ID NO: 124)); Ac-Sar₂-H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CPVNR(Aib)GC (Ac-Sar₂-(SEQ ID NO: 226)); Ac-Sar₂-H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)GC (Ac-Sar₂-(SEQ ID NO: 123)); (β-Ala)-Sar₁₀-H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)GC ((β-Ala)-Sar₁₀-(SEQ ID NO: 123)); (β-Ala)-Sar₁₀-H(D-Asp)VP-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)(D-Asp)C ((β-Ala)-Sar₁₀-(SEQ ID NO: 124)); (β-Ala)-Sar₁₀-H(D-Asp)VP-CP(1Nal)G(Aib)FWCP(tBuGly)N(HArg)P(D-Asp)C ((β-Ala)-Sar₁₀-(SEQ ID NO: 227)); Ac-Sar₂-H(D-Asp)VP-CP(1Nal)G(Aib)FWCPVNR(Aib)GC (Ac-Sar₂-(SEQ ID NO: 228)); Ac-Sar₂-H(D-Asp)VP-CPW(Aza-Gly)PFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 229)); Ac-Sar₂-H(D-Asp)VP-CPWG(Aib)F(1Nal)CPVNR(Aib)GC (Ac-Sar₂-(SEQ ID NO: 230)); (β-Ala)-Sar₁₀-H(D-Asp)VP-CPWG(Aib)F(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C ((β-Ala)-Sar₁₀-(SEQ ID NO: 231)); Ac-Sar₂-H(D-Asp)VP-CPWG(Aib)FWCP(tBuGly)NR(Aib)GC (Ac-Sar₂-(SEQ ID NO: 232)); (β-Ala)-Sar₁₀-H(D-Asp)VP-CPWG(Aib)FWCP(tBuGly)N(HArg)P(D-Asp)C ((β-Ala)-Sar₁₀-(SEQ ID NO: 233)); (β-Ala)-Sar₁₀-H(D-Asp)VP-CPWGP(HArg)WCP(tBuGly)N(HArg)P(D-Asp)C ((β-Ala)-Sar₁₀-(SEQ ID NO: 234)); (β-Ala)-Sar₁₀-H(D-Asp)(C5g)P-C(Aib)(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)(D-Asp)C ((β-Ala)-Sar₁₀-(SEQ ID NO: 125)); Ac-(β-Ala)-Sar₁₀-H(D-Asp)(C5g)P-C(Aib)(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)(D-Asp)C (Ac-(β-Ala)-Sar₁₀-(SEQ ID NO: 125)); (β-Ala)-Sar₁₀-H(D-Asp)(C5g)P-C(Aib)WG(Aib)FWCP(tBuGly)NR(Aib)(D-Asp)C ((β-Ala)-Sar₁₀-(SEQ ID NO: 235)); (β-Ala)-Sar₁₀-H(D-Asp)(C5g)P-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C ((β-Ala)-Sar₁₀-(SEQ ID NO: 126)); Ac-(β-Ala)-Sar₁₀-H(D-Asp)(C5g)P-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C (Ac-(β-Ala)-Sar₁₀-(SEQ ID NO: 126)); (β-Ala)-Sar₁₀-H(D-Asp)(C5g)P-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)GC ((β-Ala)-Sar₁₀-(SEQ ID NO: 236)); (β-Ala)-Sar₁₀-H(D-Asp)(C5g)T-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)GC ((β-Ala)-Sar₁₀-(SEQ ID NO: 237)); (β-Ala)-Sar₁₀-H(D-Asp)(C5g)T-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)N(HArg)P(D-Asp)C ((β-Ala)-Sar₁₀-(SEQ ID NO: 238)); Ac-Sar₂-A(D-Asp)DVT-CPWGPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 239)); Ac-Sar₂-A(D-Asp)DVT-CP(1Nal)GPFWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 240)); Ac-Sar₂-A(D-Asp)DVT-CP(1Nal)GPF(1Nal)CPVNRPGC (Ac-Sar₂-(SEQ ID NO: 241)); Ac-Sar₂-A(D-Asp)DVT-CP(1Nal)G(Aib)FWCP(tBuGly)N(HArg)PGC (Ac-Sar₂-(SEQ ID NO: 242)); Ac-Sar₂-H(D-Asp)VT-CP(1Nal)G(Aib)F(1Nal)CP(tBuGly)NR(Aib)GC (Ac-Sar₂-(SEQ ID NO: 243)); Ac-CANDWLCSLGWTC (Ac-(SEQ ID NO: 23)); Ac-CMNDWLCALGWTC (Ac-(SEQ ID NO: 24)); Ac-CMNDWLCSAGWTC (Ac-(SEQ ID NO: 25)); Sar₂-ACAWGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 244)); Sar₂-ACPAGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 245)); Sar₂-ACPWAPFWCPVNRPGC (Sar₂-(SEQ ID NO: 246)); Sar₂-ACPWGAFWCPVNRPGC (Sar₂-(SEQ ID NO: 247)); Sar₂-ACPWGPAWCPVNRPGC (Sar₂-(SEQ ID NO: 63)); Sar₂-ACPWGPFACPVNRPGC (Sar₂-(SEQ ID NO: 248)); Sar₂-ACPWGPFWCAVNRPGC (Sar₂-(SEQ ID NO: 249)); Sar₂-ACPWGPFWCPANRPGC (Sar₂-(SEQ ID NO: 250)); Sar₂-ACPWGPFWCPVARPGC (Sar₂-(SEQ ID NO: 81)); Sar₂-ACPWGPFWCPVNAPGC (Sar₂-(SEQ ID NO: 251)); Sar₂-ACPWGPFWCPVNRAGC (Sar₂-(SEQ ID NO: 84)); Sar₂-ACPWGPFWCPVNRPAC (Sar₂-(SEQ ID NO: 91)); Sar₂-AC(D-A1)WGPFWCPVNRPGC (Sar₂-(SEQ ID NO: 252)); Sar₂-ACP(D-Ala)GPFWCPVNRPGC (Sar₂-(SEQ ID NO: 253)); Sar₂-ACPW(D-Ala)PFWCPVNRPGC (Sar₂-(SEQ ID NO: 254)); Sar₂-ACPWG(D-Ala)FWCPVNRPGC (Sar₂-(SEQ ID NO: 255)); Sar₂-ACPWGP(D-Ala)WCPVNRPGC (Sar₂-(SEQ ID NO: 256)); Sar₂-ACPWGPF(D-Ala)CPVNRPGC (Sar₂-(SEQ ID NO: 257)); Sar₂-ACPWGPFWC(D-Ala)VNRPGC (Sar₂-(SEQ ID NO: 258)); Sar₂-ACPWGPFWCP(D-Ala)NRPGC (Sar₂-(SEQ ID NO: 259)); Sar₂-ACPWGPFWCPV(D-Ala)RPGC (Sar₂-(SEQ ID NO: 82)); Sar₂-ACPWGPFWCPVN(D-Ala)PGC (Sar₂-(SEQ ID NO: 260)); Sar₂-ACPWGPFWCPVNR(D-Ala)GC (Sar₂-(SEQ ID NO: 85)); Sar₂-ACPWGPFWCPVNRP(D-Ala)C (Sar₂-(SEQ ID NO: 92)); (β-Ala)-Sar₁₀-ACPWAPFWCAVNRPGC ((β-Ala)-Sar₁₀-(SEQ ID NO: 261)); Ac-Sar₂-A(D-Asp)VTCPWGPAWCPVNRPGC (Ac-Sar₂-(SEQ ID NO: 152)); and (β-Ala)-Sar₁₀-H(D-Asp)VPCP(1Nal)A(Aib)F(1Nal)CA(tBuGly)NR(Aib)(D-Asp)C (β-Ala)-Sar₁₀-(SEQ ID NO: 262)); or a pharmaceutically acceptable salt thereof.
 6. The peptide ligand as defined in claim 5, wherein the peptide ligand comprises an amino acid sequence selected from: (β-Ala)-Sar₁₀-ACPWGPFWCPVNRPGC ((β-Ala)-Sar₁₀-(SEQ ID NO: 52)); (β-Ala)-Sar₁₀-H(D-Asp)VP-CPWGPFWCPVNRPGC ((β-Ala)-Sar₁₀-(SEQ ID NO: 202)); Ac-Sar₂-AH(D-Asp)VP-CP(1Nal)GPFWCP(tBuGly)N(HArg)PGC (Ac-Sar₂-(SEQ ID NO: 203)); and (β-Ala)-Sar₁₀-ACPWAPFWCAVNRPGC ((β-Ala)-Sar₁₀-(SEQ ID NO: 261)); or a pharmaceutically acceptable salt thereof.
 7. The peptide ligand as defined in claim 5, wherein the peptide ligand is the free acid or a pharmaceutically acceptable salt selected from a sodium salt, a potassium salt, a calcium salt, and an ammonium salt.
 8. The peptide ligand as defined in claim 5, wherein the EphA2 is human EphA2.
 9. A pharmaceutical composition which comprises the peptide ligand as defined in claim 5, or a pharmaceutically acceptable salt thereof, in combination with one or more pharmaceutically acceptable excipients. 